Longitudinal Assessment of Diagnostic Test Performance Over the Course of Acute SARS-CoV-2 Infection.

Autor:	Smith RL; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Department of Pathobiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Carle Illinois College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Gibson LL; Division of Infectious Diseases and Immunology, Departments of Medicine and Pediatrics, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Martinez PP; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Department of Statistics, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Ke R; T-6, Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, New Mexico, USA., Mirza A; Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA., Conte M; Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA., Gallagher N; Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Conte A; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Wang L; Veterinary Diagnostic Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Fredrickson R; Veterinary Diagnostic Laboratory, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Edmonson DC; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Baughman ME; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Chiu KK; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Choi H; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Jensen TW; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Cancer Center at Illinois, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Scardina KR; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Bradley S; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Gloss SL; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Reinhart C; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Yedetore J; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA., Owens AN; Center for Clinical and Translational Research, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Broach J; UMass Memorial Medical Center, Worcester, Massachusetts, USA.; Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Barton B; Division of Biostatistics and Health Services Research, University of Massachusetts Medical School, Worcester, Massachusetts, USA.; Department of Population and Quantitative Health Sciences, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Lazar P; Division of Biostatistics and Health Services Research, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Henness D; Carle Foundation Hospital, Urbana, Illinois, USA., Young T; Carle Foundation Hospital, Urbana, Illinois, USA., Dunnett A; Carle Foundation Hospital, Urbana, Illinois, USA., Robinson ML; Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA., Mostafa HH; Division of Medical Microbiology, Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA., Pekosz A; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA., Manabe YC; Division of Infectious Diseases, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, USA., Heetderks WJ; National Institute for Biomedical Imaging and Bioengineering, Bethesda, Maryland, USA., McManus DD; Division of Cardiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA., Brooke CB; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2021 Sep 17; Vol. 224 (6), pp. 976-982.
DOI:	10.1093/infdis/jiab337
Abstrakt:	Background: Serial screening is critical for restricting spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by facilitating timely identification of infected individuals to interrupt transmission. Variation in sensitivity of different diagnostic tests at different stages of infection has not been well documented. Methods: In a longitudinal study of 43 adults newly infected with SARS-CoV-2, all provided daily saliva and nasal swabs for quantitative reverse transcription polymerase chain reaction (RT-qPCR), Quidel SARS Sofia antigen fluorescent immunoassay (FIA), and live virus culture. Results: Both RT-qPCR and Quidel SARS Sofia antigen FIA peaked in sensitivity during the period in which live virus was detected in nasal swabs, but sensitivity of RT-qPCR tests rose more rapidly prior to this period. We also found that serial testing multiple times per week increases the sensitivity of antigen tests. Conclusions: RT-qPCR tests are more effective than antigen tests at identifying infected individuals prior to or early during the infectious period and thus for minimizing forward transmission (given timely results reporting). All tests showed >98% sensitivity for identifying infected individuals if used at least every 3 days. Daily screening using antigen tests can achieve approximately 90% sensitivity for identifying infected individuals while they are viral culture positive. (© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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