Laser Microtomography for IVF Oocyte of Human.

Autor: Pogorelov AG; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia. agpogorelov@rambler.ru., Makarova NP; Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, Russia., Kalinina EA; Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, Russia., Panait AI; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia., Pogorelova VN; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia., Sukhih GT; Kulakov Research Center for Obstetrics, Gynecology, and Perinatology, Moscow, Russia.
Jazyk: angličtina
Zdroj: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2021 May; Vol. 498 (1), pp. 190-192. Date of Electronic Publication: 2021 Jun 29.
DOI: 10.1134/S1607672921030078
Abstrakt: For in vitro fertilization technology, the quality of oocytes has a direct impact on the egg fertilization and developmental competence of early embryo. The morphological criteria are used for the estimation of oocyte quality before its fertilization in vitro. To date, only one method is known to determine the maturity of oocyte. This is the routine observation with a light microscope. The aim of this article was to adapt the noninvasive quantitative laser scanning microtomography (QLSM) for the investigation of morphological features of a human oocyte in vitro. This approach was used to accumulate the Z-stack gallery of optical sections of IVF oocyte. The layer-by-layer acquisition allows the fine cytoplasmic structure imaging. Applying the QLSM Z-stack of optical sections, the cellular volume was calculated with quantitative 3D reconstruction of a human oocyte. The volume value and intracellular structure were used as novel criteria to assess the oocyte state after the stress evoked by cryopreservation procedure.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje