Hypericin-P123-photodynamic therapy in an ex vivo model as an alternative treatment approach for onychomycosis caused by Fusarium spp.

Autor: Conrado PCV; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil., Sakita KM; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil., Arita GS; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil., Gonçalves RS; Department of Chemistry, State University of Maringa, Parana, Brazil., Cesar GB; Department of Chemistry, State University of Maringa, Parana, Brazil., Caetano W; Department of Chemistry, State University of Maringa, Parana, Brazil., Hioka N; Department of Chemistry, State University of Maringa, Parana, Brazil., Voidaleski MF; Department of Pathology Basic, State Federal University of Parana, Parana, Brazil., Vicente VA; Department of Pathology Basic, State Federal University of Parana, Parana, Brazil., Svidzinski TIE; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil., Bonfim-Mendonça PS; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil., Kioshima ES; Department of Analysis Clinics and Biomedicine, State University of Maringa, Parana, Brazil. Electronic address: eskcotica@uem.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2021 Sep; Vol. 35, pp. 102414. Date of Electronic Publication: 2021 Jun 26.
DOI: 10.1016/j.pdpdt.2021.102414
Abstrakt: BackgroundFusarium has been considered an opportunistic pathogen, causing several infections in humans, including onychomycosis. In addition, a high resistance to conventional antifungals has been linked to this genus. Photodynamic Therapy (PDT), known as a non-invasive therapy, can be an alternative treatment for fungal infections, based on the excitation of a photosensitizing compound (PS) by a specific length of light, causing damage to the target. The aim of this study was to evaluate the effects of a formulation of Hypericin (Hyp) encapsulated in Pluronic™ (P123), via photodynamic therapy (PDT), on planktonic cells and biofilms in Fusarium spp. using in vitro and ex vivo assays. Materials & Methods epidemiology studies about Fusarium spp. in onychomycosis was perfomed, carried out molecular identification, compared the antifungal activity of the conventional antifungals with PDT with encapsulated Hypericin (Hyp-P123), carried out detection of reactive oxygen species, and measured the antibiofilm effect of the Hyp-P123-PDT in vitro and in an ex vivo model of onychomycosis. Results Hyp-P123-PDT exhibited a fungicidal effect in vitro with reductions ≥ 3 log 10 . ROS generation increased post-Hyp-P123-PDT in Fusarium spp. Hyp-P123-PDT showed a potent inhibitory effect on adhesion-phase and mature biofilms in vitro tests and an ex vivo model of onychomycosis (p<0.0001). Conclusion Hyp-P123-PDT had a potent effect against Fusarium spp., suggesting that photodynamic therapy with Hyp-P123 is a safe and promising treatment for onychomycosis in clinical practice.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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