Evaluation of probiotic properties of Lysinibacillus macroides under in vitro conditions and culture of Cyprinus carpio on growth parameters.

Autor: Mani SR; Department of Biotechnology, School of Aquaculture, Karpaga Vinayaga College of Engineering and Technology, Padalam, Chengalpattu, Tamil Nadu, 603308, India., Vijayan K; Department of Biotechnology, School of Aquaculture, Karpaga Vinayaga College of Engineering and Technology, Padalam, Chengalpattu, Tamil Nadu, 603308, India., Jacob JP; Department of Biotechnology, St. Joseph's College of Engineering, Sholinganallur, Chennai, Tamil Nadu, 600119, India., Vijayakumar S; Marine College, Shandong University, Weihai, 264209, People's Republic of China., Kandhasamy S; Department of Biotechnology, School of Aquaculture, Karpaga Vinayaga College of Engineering and Technology, Padalam, Chengalpattu, Tamil Nadu, 603308, India. ksivakumar76@gmail.com.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Sep; Vol. 203 (7), pp. 4705-4714. Date of Electronic Publication: 2021 Jun 29.
DOI: 10.1007/s00203-021-02452-x
Abstrakt: Cyprinus carpio is an important freshwater fish in aquaculture. It was used for the isolation of potential probiotic strain for aquaculture applications. The most dominant strain was isolated on MRS agar from the gastrointestinal (GI) of C. carpio and identified as Lysinibacillus macroides using molecular marker 16S rRNA gene. Various probiotic properties such as acid and bile tolerance and antibiotic susceptibility were analysed under in vitro conditions. Further, formulate pelletized feed using probiotic (L. macroides) in different concentrations (2, 4, 6 and 8%). Rearing of C. carpio was carried out 45 days and fed with formulated feed. The highest length (5.14 ± 0.07 cm) and weight (3.56 ± 0.07 g) of C. carpio fingerlings was recorded in the 8% LM probiotic pelletized feed, while in fingerlings fed with control showed lower in the length (3.02 ± 0.13 cm) and the weight (0.92 ± 0.04 g) on the 45th day of the experiment. Both percentage of weight gain (PWG) and specific growth rate (SGR) were significantly increased (P < 0.05) of C. carpio fingerlings fed with probiotic feed compared to control feed. Hence, the use of probiotic bacteria could be an encouraging alternative feed for future endeavours in the field of aquaculture. In conclusion, L. macroides can serve as probiotic for sustainable, competitive and promising beneficial bacteria to aquaculture industry.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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