De Novo Design of Functional Coassembling Organic-Inorganic Hydrogels for Hierarchical Mineralization and Neovascularization.

Autor: Okesola BO; Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.; School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K., Mendoza-Martinez AK; Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.; School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K., Cidonio G; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, U.K.; Center for Life Nano- & Neuro- Science (CL2NS), Fondazione Istituto Italiano di Tecnologia, 00161 Rome, Italy., Derkus B; Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.; School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K.; Department of Chemistry, Faculty of Science, Ankara University, 06560 Ankara, Turkey., Boccorh DK; Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow G1 1RD, U.K., Osuna de la Peña D; School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K., Elsharkawy S; Centre for Oral, Clinical, and Translational Sciences, Faculty of Dentistry, Oral, and Craniofacial Sciences, King's College London, London SE1 1UL, U.K., Wu Y; School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, U.K.; Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, U.K., Dawson JI; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, U.K., Wark AW; Department of Pure and Applied Chemistry, Technology and Innovation Centre, University of Strathclyde, Glasgow G1 1RD, U.K., Knani D; Department of Biotechnology Engineering, ORT Braude College, Karmiel 2161002, Israel., Adams DJ; School of Chemistry, College of Science and Engineering, University of Glasgow, Glasgow G12 8QQ, U.K., Oreffo ROC; Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of Southampton, Southampton SO16 6YD, U.K., Mata A; Institute of Bioengineering, Queen Mary University of London, London E1 4NS, U.K.; School of Engineering and Materials Science, Queen Mary University of London, London E1 4NS, U.K.; School of Pharmacy, University of Nottingham, Nottingham NG7 2RD, U.K.; Biodiscovery Institute, University of Nottingham, Nottingham NG7 2RD, U.K.; Department of Chemical and Environmental Engineering, University of Nottingham, Nottingham NG7 2RD, U.K.
Jazyk: angličtina
Zdroj: ACS nano [ACS Nano] 2021 Jul 27; Vol. 15 (7), pp. 11202-11217. Date of Electronic Publication: 2021 Jun 28.
DOI: 10.1021/acsnano.0c09814
Abstrakt: Synthetic nanostructured materials incorporating both organic and inorganic components offer a unique, powerful, and versatile class of materials for widespread applications due to the distinct, yet complementary, nature of the intrinsic properties of the different constituents. We report a supramolecular system based on synthetic nanoclay (Laponite, Lap ) and peptide amphiphiles (PAs, PAH3 ) rationally designed to coassemble into nanostructured hydrogels with high structural integrity and a spectrum of bioactivities. Spectroscopic and scattering techniques and molecular dynamic simulation approaches were harnessed to confirm that PAH3 nanofibers electrostatically adsorbed and conformed to the surface of Lap nanodisks. Electron and atomic force microscopies also confirmed an increase in diameter and surface area of PAH3 nanofibers after coassembly with Lap . Dynamic oscillatory rheology revealed that the coassembled PAH3-Lap hydrogels displayed high stiffness and robust self-healing behavior while gas adsorption analysis confirmed a hierarchical and heterogeneous porosity. Furthermore, this distinctive structure within the three-dimensional (3D) matrix provided spatial confinement for the nucleation and hierarchical organization of high-aspect ratio hydroxyapatite nanorods into well-defined spherical clusters within the 3D matrix. Applicability of the organic-inorganic PAH3-Lap hydrogels was assessed in vitro using human bone marrow-derived stromal cells (hBMSCs) and ex vivo using a chick chorioallantoic membrane (CAM) assay. The results demonstrated that the organic-inorganic PAH3-Lap hydrogels promote human skeletal cell proliferation and, upon mineralization, integrate with the CAM, are infiltrated by blood vessels, stimulate extracellular matrix production, and facilitate extensive mineral deposition relative to the controls.
Databáze: MEDLINE