Static Magnetic Stimulation Induces Changes in the Oxidative Status and Cell Viability Parameters in a Primary Culture Model of Astrocytes.

Autor: da Costa CC; NeuroCell Laboratory, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Martins LAM; Department of Physiology, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, Bairro Centro Histórico, Porto Alegre, Rio Grande do Sul, 90050-170, Brasil., Koth AP; NeuroCell Laboratory, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Ramos JMO; NeuroCell Laboratory, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Guma FTCR; Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Bairro Santa Cecília, Porto Alegre, Rio Grande do Sul, 90035-000, Brasil., de Oliveira CM; Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Bairro Santa Cecília, Porto Alegre, Rio Grande do Sul, 90035-000, Brasil., Pedra NS; Laboratory of Neurochemistry, Inflammation and Cancer, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Fischer G; Laboratory of Virology and Immunology, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Helena ES; Department of Physiological Sciences, Universidade Federal de Rio Grande Avenida Itália, Km 8, Bairro Carreiros, Rio Grande, Rio Grande do Sul, 96203-900, Brasil., Gioda CR; Department of Physiological Sciences, Universidade Federal de Rio Grande Avenida Itália, Km 8, Bairro Carreiros, Rio Grande, Rio Grande do Sul, 96203-900, Brasil., Sanches PRS; Laboratory of the Research and Development Service in Biomedical Engineering- Hospital de Clínicas de Porto Alegre Rua Ramiro Barcelos, 2350- Bairro Santa Cecília, Porto Alegre-RS, 90035-903, Brasil., Junior ASV; Institute of Biological Science, Universidade Federal do Rio Grande Avenida Itália, Km 8, Bairro Carreiros, Rio Grande, Rio Grande do Sul, 96203-900, Brasil., Soares MSP; Laboratory of Neurochemistry, Inflammation and Cancer, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Spanevello RM; Laboratory of Neurochemistry, Inflammation and Cancer, Post-Graduate Program in Biochemistry and Bioprospection, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., Gamaro GD; NeuroCell Laboratory, Universidade Federal de Pelotas Campus Universitário, S/N, Capão do Leão-RS, 96160-000, Brasil., de Souza ICC; Coordinator of NeuroCell Laboratory, Laboratory of Histology, Department of Morphology, Post-Graduate Program in Biochemistry and Bioprospection, Universidade Federal de Pelotas Avenida Duque de Caxias, 250, 96030-000, Pelotas, Rio Grande do Sul, Brasil. izabel.souza@ufpel.edu.br.
Jazyk: angličtina
Zdroj: Cell biochemistry and biophysics [Cell Biochem Biophys] 2021 Dec; Vol. 79 (4), pp. 873-885. Date of Electronic Publication: 2021 Jun 27.
DOI: 10.1007/s12013-021-01015-7
Abstrakt: Astrocytes play an important role in the central nervous system function and may contribute to brain plasticity response during static magnetic fields (SMF) brain therapy. However, most studies evaluate SMF stimulation in brain plasticity while few studies evaluate the consequences of SMF at the cellular level. Thus, we here evaluate the effects of SMF at 305 mT (medium-intensity) in a primary culture of healthy/normal cortical astrocytes obtained from neonatal (1 to 2-day-old) Wistar rats. After reaching confluence, cells were daily subjected to SMF stimulation for 5 min, 15 min, 30 min, and 40 min during 7 consecutive days. Oxidative stress parameters, cell cycle, cell viability, and mitochondrial function were analyzed. The antioxidant capacity was reduced in groups stimulated for 5 and 40 min. Although no difference was observed in the enzymatic activity of superoxide dismutase and catalase or the total thiol content, lipid peroxidation was increased in all stimulated groups. The cell cycle was changed after 40 min of SMF stimulation while 15, 30, and 40 min led cells to death by necrosis. Mitochondrial function was reduced after SMF stimulation, although imaging analysis did not reveal substantial changes in the mitochondrial network. Results mainly revealed that SMF compromised healthy astrocytes' oxidative status and viability. This finding reveals how important is to understand the SMF stimulation at the cellular level since this therapeutic approach has been largely used against neurological and psychiatric diseases.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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