Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device.

Autor: Brandão FAS; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Alves KA; Ovid Research Company, Berkeley, CA, USA., de Brito DCC; Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Pereira LMC; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., de Morais GB; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Ñaupas LVS; Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., de Souza SS; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Alves BG; Ovid Research Company, Berkeley, CA, USA., Rodrigues APR; Laboratory of Manipulation of Oocytes and Ovarian Preantral Follicles (LAMOFOPA), Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Teixeira DÍA; Diagnostic Imaging Laboratory Applied to Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil.
Jazyk: angličtina
Zdroj: Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2021 Aug; Vol. 56 (8), pp. 1156-1161. Date of Electronic Publication: 2021 Jul 14.
DOI: 10.1111/rda.13979
Abstrakt: The present study evaluated the effect of Ovarian Tissue Cryosystem (OTC) on follicular morphology and density, as well as on stromal cell density of vitrified canine ovarian tissue. Canine ovarian fragments collected from adult female dogs in stages of the random oestrous cycle were fixed (FC, fresh control) or vitrified (VIT) with an OTC device. After vitrification and warming, the fragments were fixed for histological analysis. Overall, the mean percentage of normal pre-antral follicles decreased after vitrification procedure (FC: 74.5% ± 1.6% vs. VIT: 52.05% ± 1.5%). Although the rates of normal primordial (71.1% ± 1.8%) and secondary (0.7% ± 0.4%) follicles vitrified showed a reduction (p < .05), vitrification using OTC showed considerable preservation of follicles, when compared to the fresh control (81.1% ± 1.5% and 2.3% ± 0.6%, respectively). The mean follicular density was maintained after vitrification (FC: 199.65 ± 12.8 vs. VIT: 199.68 ± 10.8), whereas the stromal cell density decreased in the VIT group. Based on the results, we recommend the use of OTC for vitrification of canine ovarian tissue.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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