Detection of porcine circovirus type 2 (PCV2) in the Philippines and the complexity of PCV2-associated disease diagnosis.

Autor: Dela Cruz A; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia. agnes.delacruz@uqconnect.edu.au., Palmieri C; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia., Azul R; Bureau of Animal Industry, Department of Agriculture, Quezon City, Philippines., Legaspi C; Bureau of Animal Industry, Department of Agriculture, Quezon City, Philippines., Lola S; University of the Philippines, Los Baños, Laguna, Philippines., Barnes TS; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia.; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia., Parke CR; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia., Turni C; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia., Alawneh JI; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia., Baluyut AS; Provincial Veterinary Office of Pampanga, San Fernando, Philippines., Basinang VG; Provincial Veterinary Office of Bulacan, Malolos, Philippines., David JE; Bureau of Animal Industry, Department of Agriculture, Quezon City, Philippines., de Castro RO; Livestock Research Division PCAARRD-DOST, Los Baños, Laguna, Philippines., Domingo R; Bureau of Animal Industry, Department of Agriculture, Quezon City, Philippines., Francisco E; Provincial Veterinary Office of Bulacan, Malolos, Philippines., Ignacio C; Provincial Veterinary Office of Pampanga, San Fernando, Philippines., Lapuz EL Jr; Department of Agriculture Region 3, San Fernando, Pampanga, Philippines., Mananggit MR; Department of Agriculture Region 3, San Fernando, Pampanga, Philippines., Retes L; Bureau of Animal Industry, Department of Agriculture, Quezon City, Philippines., Villar EC; Livestock Research Division PCAARRD-DOST, Los Baños, Laguna, Philippines., Blackall PJ; The University of Queensland, Queensland Alliance for Agriculture and Food Innovation, Brisbane, QLD, Australia., Meers J; The University of Queensland, School of Veterinary Science, Gatton, QLD, Australia.
Jazyk: angličtina
Zdroj: Tropical animal health and production [Trop Anim Health Prod] 2021 Jun 25; Vol. 53 (3), pp. 371. Date of Electronic Publication: 2021 Jun 25.
DOI: 10.1007/s11250-021-02823-y
Abstrakt: Porcine circovirus type 2 (PCV2), an important pig viral pathogen, can cause porcine circovirus-associated disease (PCVAD), resulting in economic losses associated with decreased growth and mortalities. The diagnosis of PCVAD is complex requiring clinical, pathological and virological approaches. This study assessed PCV2 infection using histopathology and immunohistochemistry (IHC) on tissue samples and quantitative polymerase chain reaction (qPCR) on serum samples from 47 grower-finisher pigs allocated in three clinical groups in the Philippines. Typical PCV2 histopathological lesions were observed in mediastinal lymph nodes (MLN) of eight of 47 pigs. Lymphoid depletion was seen in all eight pigs and granulomatous inflammation in one of these pigs. Four of these eight pigs were PCV2 positive by both IHC and qPCR. IHC revealed PCV2 antigen in 8 pigs in at least one of the following tissues: MLN (5/8), spleen (3/8), tonsils (4/8) and lungs (5/8). PCV2 antigen was observed in 3/8 MLN with lymphoid depletion and in one MLN with depletion and granulomatous inflammation. The qPCR test showed that 33 sera had a non-detectable level, twelve had < 10 6 and two had > 10 6 PCV2 DNA copies/ml serum. One pig with lymphoid depletion had > 10 6 PCV2 DNA copies/ml serum, and another pig without MLN lesions also had > 10 6 PCV2 DNA copies/ml serum. These findings suggest that PCVAD is present in the Philippines and confirm the challenges of PCVAD diagnosis as different patterns of results were obtained from the different tests.
Databáze: MEDLINE
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