High concentrations of β-hydroxybutyrate alter the kinetics of bovine spermatozoa.

Autor: Missio D; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil., Dos Santos Brum D; Laboratory of Biotechnology of Reproduction - BIOTECH, Federal University of Pampa, Uruguaiana, Brazil., Dalle Laste Dacampo L; Laboratory of Biotechnology of Reproduction - BIOTECH, Federal University of Pampa, Uruguaiana, Brazil., Weber Santos Cibin F; Laboratory of Biotechnology of Reproduction - BIOTECH, Federal University of Pampa, Uruguaiana, Brazil., Silveira Mesquita F; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, Brazil., Germano Ferst J; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, Brazil., Fiordalisi G; Department of Animal Science, Santa Catarina State University, Chapecó, Brazil., Dias Gonçalves PB; Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, Brazil.; Molecular and Integrative Physiology of Reproduction Laboratory, MINT, Federal University of Pampa, Uruguaiana, Brazil., Ferreira R; Department of Animal Science, Santa Catarina State University, Chapecó, Brazil.
Jazyk: angličtina
Zdroj: Andrologia [Andrologia] 2021 Sep; Vol. 53 (8), pp. e14148. Date of Electronic Publication: 2021 Jun 25.
DOI: 10.1111/and.14148
Abstrakt: Postpartum cows, mainly with metabolic diseases, such as ketosis, usually experience an increased number of services per conception. During ketosis, high concentrations of β-hydroxybutyrate (BHBA) in follicular, uterine and oviductal fluid have been considered to cause subfertility in cows. However, the effect of sperm exposure to an environment with high BHBA concentration is not known. This study investigated the influence of high levels of BHBA on kinetics, oxidative status and morphology of bovine spermatozoa. To assess the effect of BHBA after sperm selection, bovine spermatozoa were incubated (180 min) with different BHBA concentrations: 0 (Control), 0.8, 2.4 or 5 mM. Sperm kinetics was evaluated after 30, 60, 120 and 180 min, and oxidative status and morphology were analysed at 180 min. Oxidative status was evaluated through the production of reactive oxidative species (ROS), total antioxidant capacity and lipid peroxidation. High concentrations of BHBA decreased the curvilinear velocity, straight line velocity, mean path velocity, linearity, straightness and hyperactivity of spermatozoa. However, there was no effect of BHBA on oxidative and antioxidant capacity as well as on sperm morphology. In conclusion, exposure of bovine spermatozoa to high levels of BHBA impairs sperm kinetics without altering oxidative and antioxidant mechanisms.
(© 2021 Wiley-VCH GmbH.)
Databáze: MEDLINE
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