Effects of thymosin β4-derived peptides on migration and invasion of ovarian cancer cells.

Autor: Yoon HJ; Department of Medicine, Pusan National University School of Medicine, Pusan National University Hospital, Busan, South Korea., Oh YL; Department of Obstetrics and Gynecology, Kosin University College of Medicine, Busan, South Korea., Ko EJ; Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, South Korea., Kang A; Department of Biochemistry, Kosin University College of Medicine, Busan, South Korea., Eo WK; Department of Internal Medicine, Kyung Hee University College of Medicine, Seoul, South Korea., Kim KH; Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Pusan National University Hospital, Busan, South Korea., Lee JY; Department of Obstetrics and Gynecology, Konkuk University School of Medicine, Seoul, South Korea., Kim A; Department of Obstetrics and Gynecology, Chicago Medical School, Rosalind Franklin University of Medicine and Science, Vernon Hills, North Chicago, IL, USA., Chun S; Department of Obstetrics and Gynecology, Inje University Haeundae Paik Hospital, Busan, South Korea., Kim H; Department of Obstetrics and Gynecology, Hallym University Medical Center, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, Seoul, South Korea., Ock MS; Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, South Korea., Cha HJ; Department of Parasitology and Genetics, Kosin University College of Medicine, Busan, South Korea. hcha@kosin.ac.kr.
Jazyk: angličtina
Zdroj: Genes & genomics [Genes Genomics] 2021 Aug; Vol. 43 (8), pp. 987-993. Date of Electronic Publication: 2021 Jun 25.
DOI: 10.1007/s13258-021-01127-7
Abstrakt: Background: Thymosin β4 (Tβ4) is a highly conserved actin binding protein associated with the metastatic potential of tumor cells by stimulating cell migration. The role of Tβ4 and its derived fragment peptides in migration of ovarian cancer cells has not been studied.
Objective: To analyze the effects of Tβ4 and its derived fragment peptides on ovarian cancer cell migration and invasion, we applied Tβ4 and three Tβ4-derived synthetic peptides to SKOV3 ovarian cancer cells.
Method: The migration and invasion of SKOV3 cells treated with Tβ4(1-43), Tβ4(1-15), Tβ4(12-26), Tβ4(23-), and untreated control were analyzed by in vitro migration and invasion assay with transwell plate. Cell proliferation assay was conducted to identify the effect of Tβ4 and its derived peptide on SKOV3 cell proliferation. The expression of Tβ4 related proteins related with cell proliferation was analyzed by Western blot after treatment with Tβ4 and its derived peptides.
Results: Cell migration and invasion were significantly increased in Tβ4 peptide-treated SKOV3 cells compared with untreated control. All three Tβ4-derived fragment peptides including those without an actin binding site significantly stimulated migration and invasion of SKOV3 cells. Tβ4 and its derived peptide significantly stimulated SKOV3 cell proliferation and up-regulated the expression of RACK-1 protein.
Conclusions: The Tβ4 peptide and all of its derived fragment peptides including those without an actin binding motif stimulate migration and invasion of SKOV3 ovarian cancer cells. All peptides significantly increased RACK-1 expression and cell proliferation of SKOV3 cells. These results suggest that Tβ4 stimulates migration and invasion of SKOV3 cells by stimulation of cell proliferation through up-regulation of RACK-1 protein.
(© 2021. The Genetics Society of Korea.)
Databáze: MEDLINE
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