ADF and cofilin-1 collaborate to promote cortical actin flow and the leader bleb-based migration of confined cells.

Autor:	Ullo MF; Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, United States., Logue JS; Department of Regenerative and Cancer Cell Biology, Albany Medical College, Albany, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Jun 25; Vol. 10. Date of Electronic Publication: 2021 Jun 25.
DOI:	10.7554/eLife.67856
Abstrakt:	Melanoma cells have been shown to undergo fast amoeboid (leader bleb-based) migration, requiring a single large bleb for migration. In leader blebs, is a rapid flow of cortical actin that drives the cell forward. Using RNAi, we find that co-depleting cofilin-1 and actin depolymerizing factor (ADF) led to a large increase in cortical actin, suggesting that both proteins regulate cortical actin. Furthermore, severing factors can promote contractility through the regulation of actin architecture. However, RNAi of cofilin-1 but not ADF led to a significant decrease in cell stiffness. We found cofilin-1 to be enriched at leader bleb necks, whereas RNAi of cofilin-1 and ADF reduced bleb sizes and the frequency of motile cells. Strikingly, cells without cofilin-1 and ADF had blebs with abnormally long necks. Many of these blebs failed to retract and displayed slow actin turnover. Collectively, our data identifies cofilin-1 and ADF as actin remodeling factors required for fast amoeboid migration. Competing Interests: MU, JL No competing interests declared (© 2021, Ullo and Logue.)
Databáze:	MEDLINE
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