Lower Ventromedial Prefrontal Cortex Glutamate Levels in Patients With Obsessive-Compulsive Disorder.

Autor:	Batistuzzo MC; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil.; Department of Methods and Techniques in Psychology, Pontifical Catholic University, São Paulo, Brazil., Sottili BA; Laboratory of Magnetic Resonance (LIM44), Department and Institute of Radiology, University of São Paulo (InRad-FMUSP), São Paulo, Brazil., Shavitt RG; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Lopes AC; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Cappi C; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., de Mathis MA; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Pastorello B; Laboratory of Magnetic Resonance (LIM44), Department and Institute of Radiology, University of São Paulo (InRad-FMUSP), São Paulo, Brazil., Diniz JB; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Silva RMF; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Miguel EC; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil., Hoexter MQ; Department & Institute of Psychiatry, University of São Paulo Medical School, São Paulo, Brazil.; Laboratório Interdisciplinar de Neurociências Clínicas (LiNC), Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil., Otaduy MC; Laboratory of Magnetic Resonance (LIM44), Department and Institute of Radiology, University of São Paulo (InRad-FMUSP), São Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Frontiers in psychiatry [Front Psychiatry] 2021 Jun 08; Vol. 12, pp. 668304. Date of Electronic Publication: 2021 Jun 08 (Print Publication: 2021).
DOI:	10.3389/fpsyt.2021.668304
Abstrakt:	Background: Recent studies using magnetic resonance spectroscopy ( 1 H-MRS) indicate that patients with obsessive-compulsive disorder (OCD) present abnormal levels of glutamate (Glu) and gamma aminobutyric acid (GABA) in the frontal and striatal regions of the brain. These abnormalities could be related to the hyperactivation observed in cortico-striatal circuits of patients with OCD. However, most of the previous 1 H-MRS studies were not capable of differentiating the signal from metabolites that overlap in the spectrum, such as Glu and glutamine (Gln), and referred to the detected signal as the composite measure-Glx (sum of Glu and Gln). In this study, we used a two-dimensional JPRESS 1 H-MRS sequence that allows the discrimination of overlapping metabolites by observing the differences in J-coupling, leading to higher accuracy in the quantification of all metabolites. Our objective was to identify possible alterations in the neurometabolism of OCD, focusing on Glu and GABA, which are key neurotransmitters in the brain that could provide insights into the underlying neurochemistry of a putative excitatory/inhibitory imbalance. Secondary analysis was performed including metabolites such as Gln, creatine (Cr), N-acetylaspartate, glutathione, choline, lactate, and myo-inositol. Methods: Fifty-nine patients with OCD and 42 healthy controls (HCs) underwent 3T 1 H-MRS in the ventromedial prefrontal cortex (vmPFC, 30 × 25 × 25 mm 3 ). Metabolites were quantified using ProFit (version 2.0) and Cr as a reference. Furthermore, Glu/GABA and Glu/Gln ratios were calculated. Generalized linear models (GLMs) were conducted using each metabolite as a dependent variable and age, sex, and gray matter fraction (fGM) as confounding factors. GLM analysis was also used to test for associations between clinical symptoms and neurometabolites. Results: The GLM analysis indicated lower levels of Glu/Cr in patients with OCD ( z = 2.540; p = 0.011). No other comparisons reached significant differences between groups for all the metabolites studied. No associations between metabolites and clinical symptoms were detected. Conclusions: The decreased Glu/Cr concentrations in the vmPFC of patients with OCD indicate a neurochemical imbalance in the excitatory neurotransmission that could be associated with the neurobiology of the disease and may be relevant for the pathophysiology of OCD. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer JO'N declared a shared consortium with several of the authors, MB, MH, JD, and EM, at time of review. (Copyright © 2021 Batistuzzo, Sottili, Shavitt, Lopes, Cappi, de Mathis, Pastorello, Diniz, Silva, Miguel, Hoexter and Otaduy.)
Databáze:	MEDLINE
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