Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry.

Autor:	Strutt R; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK., Hindley JW; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK., Gregg J; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk., Booth PJ; FabriCELL, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK.; Department of Chemistry, King's College London SE1 1DB London UK., Harling JD; Medicinal Chemistry, GSK Gunnels Wood Road, Stevenage SG1 2NY UK., Law RV; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK., Friddin MS; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk.; Dyson School of Design Engineering, Imperial College London Imperial College Road SW7 2AZ UK m.friddin@imperial.ac.uk., Ces O; Department of Chemistry, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK o.ces@imperial.ac.uk.; Institute of Chemical Biology, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK.; FabriCELL, Imperial College London, Molecular Sciences Research Hub Shepherd's Bush London W12 0BZ UK.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2021 Jan 04; Vol. 12 (6), pp. 2138-2145. Date of Electronic Publication: 2021 Jan 04.
DOI:	10.1039/d0sc03889j
Abstrakt:	Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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