Multiplexed optical barcoding of cells via photochemical programming of bioorthogonal host-guest recognition.

Autor:	Som A; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in., Pahwa M; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in., Bawari S; Tata Institute of Fundamental Research 36/P, Gopanpally Village Hyderabad 500046 India., Saha ND; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in., Sasmal R; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in., Bosco MS; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in., Mondal J; Tata Institute of Fundamental Research 36/P, Gopanpally Village Hyderabad 500046 India., Agasti SS; New Chemistry Unit, Chemistry & Physics of Materials Unit, School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR) Bangalore Karnataka 560064 India sagasti@jncasr.ac.in.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2021 Feb 19; Vol. 12 (15), pp. 5484-5494. Date of Electronic Publication: 2021 Feb 19.
DOI:	10.1039/d0sc06860h
Abstrakt:	Modern chemical and biological studies are undergoing a paradigm shift, where understanding the fate of individual cells, in an apparently homogeneous population, is becoming increasingly important. This has inculcated a growing demand for developing strategies that label individual cells with unique fluorescent signatures or barcodes so that their spatiotemporal trajectories can be mapped in real time. Among various approaches, light-regulated methods employing photocaged fluorophores have received particular attention, owing to their fine spatiotemporal control over labelling. However, their multiplexed use to barcode large numbers of cells for interrogating cellular libraries or complex tissues remains inherently challenging, due to the lack of multiple spectrally distinct photoactivated states in the currently available photocaged fluorophores. We report here an alternative multiplexable strategy based on optically controlled host-guest recognition in the cucurbit[7]uril (CB[7]) system that provides spatial control over the positioning of fluorophores to generate distinct barcodes in 'user-defined' cells. Using a combination of three spectrally distinct CB[7]-conjugated fluorophores and by sequentially performing cycles of photoactivation and fluorophore encoding, we demonstrate 10-color barcoding in microtubule-targeted fixed cells as well as 7-color barcoding in cell surface glycan targeted live MCF7 cells. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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