Mosquito metallomics reveal copper and iron as critical factors for Plasmodium infection.

Autor: Maya-Maldonado K; Departamento de Infectómica y Patogénesis Molecular, Cinvestav, Ciudad de México, México.; Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México., Cardoso-Jaime V; Departamento de Infectómica y Patogénesis Molecular, Cinvestav, Ciudad de México, México.; Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México., González-Olvera G; Unidad Colaborativa para Bioensayos Entomológicos, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán, México., Osorio B; Departamento de Fisiología, Biofísica y Neurociencias, Cinvestav, Ciudad de México, México., Recio-Tótoro B; Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México.; Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México., Manrique-Saide P; Unidad Colaborativa para Bioensayos Entomológicos, Campus de Ciencias Biológicas y Agropecuarias, Universidad Autónoma de Yucatán, Mérida, Yucatán, México., Rodríguez-Sánchez IP; Laboratorio de Fisiología Molecular y Estructural, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León, México., Lanz-Mendoza H; Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, Morelos, México., Missirlis F; Departamento de Fisiología, Biofísica y Neurociencias, Cinvestav, Ciudad de México, México., Hernández-Hernández FC; Departamento de Infectómica y Patogénesis Molecular, Cinvestav, Ciudad de México, México.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2021 Jun 23; Vol. 15 (6), pp. e0009509. Date of Electronic Publication: 2021 Jun 23 (Print Publication: 2021).
DOI: 10.1371/journal.pntd.0009509
Abstrakt: Iron and copper chelation restricts Plasmodium growth in vitro and in mammalian hosts. The parasite alters metal homeostasis in red blood cells to its favor, for example metabolizing hemoglobin to hemozoin. Metal interactions with the mosquito have not, however, been studied. Here, we describe the metallomes of Anopheles albimanus and Aedes aegypti throughout their life cycle and following a blood meal. Consistent with previous reports, we found evidence of maternal iron deposition in embryos of Ae. aegypti, but less so in An. albimanus. Sodium, potassium, iron, and copper are present at higher concentrations during larval developmental stages. Two An. albimanus phenotypes that differ in their susceptibility to Plasmodium berghei infection were studied. The susceptible white stripe (ws) phenotype was named after a dorsal white stripe apparent during larval stages 3, 4, and pupae. During larval stage 3, ws larvae accumulate more iron and copper than the resistant brown stripe (bs) phenotype counterparts. A similar increase in copper and iron accumulation was also observed in the susceptible ws, but not in the resistant bs phenotype following P. berghei infection. Feeding ws mosquitoes with extracellular iron and copper chelators before and after receiving Plasmodium-infected blood protected from infection and simultaneously affected follicular development in the case of iron chelation. Unexpectedly, the application of the iron chelator to the bs strain reverted resistance to infection. Besides a drop in iron, iron-chelated bs mosquitoes experienced a concomitant loss of copper. Thus, the effect of metal chelation on P. berghei infectivity was strain-specific.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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