| Autor: |
Dixit KK; ICMR-National Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India.; Faculty of Health and Biological Sciences, Symbiosis International (Deemed University), Pune, India., Ramesh V; Department of Dermatology and STD, Safdarjung Hospital, New Delhi, India., Upadhyay S; Department of Medicine, Institute of Medical Sciences, Banaras Hindu Universitygrid.411507.6, Varanasi, Uttar Pradesh, India., Singh AK; Department of Medicine, Institute of Medical Sciences, Banaras Hindu Universitygrid.411507.6, Varanasi, Uttar Pradesh, India., Singh OP; Department of Medicine, Institute of Medical Sciences, Banaras Hindu Universitygrid.411507.6, Varanasi, Uttar Pradesh, India., Sundar S; Department of Medicine, Institute of Medical Sciences, Banaras Hindu Universitygrid.411507.6, Varanasi, Uttar Pradesh, India., Singh R; ICMR-National Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India., Salotra P; ICMR-National Institute of Pathology, Safdarjung Hospital Campus, New Delhi, India. |
| Abstrakt: |
The countries in the Indian subcontinent have reported a dramatic decline in visceral leishmaniasis (VL) cases. However, the presence of the parasite reservoir in the form of post-kala-azar dermal leishmaniasis (PKDL), a dermal sequel of VL, is a hurdle in attaining VL elimination. Presently employed clinical specimens for the diagnosis of PKDL include skin biopsy specimens and slit skin smears. In this study, the use of blood as a clinical specimen was investigated in different manifestations of PKDL in India. This is a bicentric study (National Institute of Pathology, Indian Council of Medical Research [ICMR], New Delhi, and Institute of Medical Sciences [IMS], Banaras Hindu University, Varanasi), with 215 participants (120 PKDL patients and 95 controls). Highly sensitive quantitative real-time PCR (Q-PCR) and field-deployable loop-mediated isothermal amplification (LAMP) were employed using blood samples for diagnosis. Promising sensitivities of 77.50% (95% confidence interval [CI], 69.24 to 84.05%) for Q-PCR and 70.83% (95% CI, 62.16 to 78.22%) for LAMP were obtained for the diagnosis of PKDL. Further, enhanced sensitivities of 83.33% (95% CI, 71.28 to 90.98%) and 77.78% (95% CI, 65.06 to 86.80%) for Q-PCR and LAMP, respectively, were recorded for the detection of macular cases. The study revealed an inverse correlation between the parasite load estimated in slit and blood samples, thereby favoring the use of blood for the diagnosis of the macular variant, which may be missed due to scant parasite loads in the slit. This study is the first to propose the promising potential of blood as a clinical specimen for accurate diagnosis of PKDL, which would aid in fast-tracking VL elimination. |
