Variation in bovine leptin gene affects milk fatty acid composition in New Zealand Holstein Friesian  ×  Jersey dairy cows.

Autor: Haruna IL; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand., Zhou H; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand., Hickford JGH; Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln 7647, New Zealand.
Jazyk: angličtina
Zdroj: Archives animal breeding [Arch Anim Breed] 2021 Jun 07; Vol. 64 (1), pp. 245-256. Date of Electronic Publication: 2021 Jun 07 (Print Publication: 2021).
DOI: 10.5194/aab-64-245-2021
Abstrakt: Leptin is a protein hormone secreted from white adipose tissue. It regulates food/feed intake, body weight, immune function and reproduction. In our investigation, the polymerase chain reaction (PCR) amplification coupled with single-strand conformational polymorphism (SSCP) analysis was used to reveal variation in bovine leptin gene ( LEP ) in New Zealand (NZ) Holstein Friesian  ×  Jersey (HF  ×  J) dairy cows. Subsequent sequence analysis of a 430 bp amplicon spanning the entirety of exon 3 and part of the intron 2 region revealed three variant sequences ( A 3 , B 3 and C 3 ) containing a total of five nucleotide substitutions, all of which have been reported previously. Using general linear mixed-effect model analyses, the presence of variant A 3 (the most common variant) was associated with a decreased level of C15:1, C18:1 trans -11, C18:1 all trans , C18:2 trans -9, cis -12, C22:0 and C24:0 levels but increased levels of C12:1 and C13:0 iso ( p < 0.05 ). Variant B 3 was associated with reduced levels of C6:0, C8:0, C11:0, C13:0 and C20:0 but increased C17:0 iso and C24:0 levels ( p < 0.05 ). Variant C 3 was associated with decreased C17:0 iso levels but increased C20:0 ( p < 0.05 ) levels. In a genotype model, the A 3 B 3 genotype was associated with increased levels of C22:0 and C24:0 but decreased C8:0, C10:0, C11:0, C13:0, C15:0 and grouped medium-chain fatty acid (MCFA) levels ( p < 0.05 ). Genotype A 3 C 3 was found to be associated with decreased levels of C10:0, C11:0, C13:0 and grouped MCFA ( p < 0.05 ). This is the first report of findings of this kind in NZ HF  ×  J cows, and they suggest that variation in exon 3 of bovine leptin gene could be explored as a means of decreasing the concentration of saturated fatty acids in milk.
Competing Interests: The authors declare that they have no conflict of interest.
(Copyright: © 2021 Ishaku Lemu Haruna et al.)
Databáze: MEDLINE
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