Role of bacterial motility in differential resistance mechanisms of silver nanoparticles and silver ions.

Autor: Stabryla LM; Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA, USA. lms162@pitt.edu., Johnston KA; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA., Diemler NA; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA., Cooper VS; Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA, USA., Millstone JE; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, USA.; Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA.; Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA, USA., Haig SJ; Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA, USA.; Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA., Gilbertson LM; Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA, USA. leanne.gilbertson@pitt.edu.; Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA. leanne.gilbertson@pitt.edu.
Jazyk: angličtina
Zdroj: Nature nanotechnology [Nat Nanotechnol] 2021 Sep; Vol. 16 (9), pp. 996-1003. Date of Electronic Publication: 2021 Jun 21.
DOI: 10.1038/s41565-021-00929-w
Abstrakt: Unlike conventional antimicrobials, the study of bacterial resistance to silver nanoparticles (AgNPs) remains in its infancy and the mechanism(s) through which it evolves are limited and inconclusive. The central question remains whether bacterial resistance is driven by the AgNPs, released Ag(I) ions or a combination of these and other factors. Here, we show a specific resistance in an Escherichia coli K-12 MG1655 strain to subinhibitory concentrations of AgNPs, and not Ag(I) ions, as indicated by a statistically significant greater-than-twofold increase in the minimum inhibitory concentration occurring after eight repeated passages that was maintained after the AgNPs were removed and reintroduced. Whole-population genome sequencing identified a cusS mutation associated with the heritable resistance that possibly increased silver ion efflux. Finally, we rule out the effect of particle aggregation on resistance and suggest that the mechanism of resistance may be enhanced or mediated by flagellum-based motility.
(© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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