| Autor: |
Boriskin YS; Institute for Viral Preparations, Moscow, U.S.S.R., Steinberg LL, Dorofeeva LV, Zasorina IN, Barkova EP |
| Jazyk: |
angličtina |
| Zdroj: |
Archives of virology [Arch Virol] 1988; Vol. 101 (1-2), pp. 131-6. |
| DOI: |
10.1007/BF01314658 |
| Abstrakt: |
Supplementation of culture medium with MgSO4 or Na2SO4 in millimolar concentrations caused an enhanced measles virus (MV) yield from cultured quail embryo cells. MgSO4 at 25-50 mM concentrations exhibited the most pronounced and consistent stimulatory effect. MV infectivity increases ranged from 2- to 200-fold; the effect was highly reproducible for stationary monolayer, roller or microcarrier-grown cell culture types. MgSO4 also improved MV plaque development and caused MV plaque size enlargement on Vero cell monolayers. At mM concentrations MgSO4 was not operative as a MV thermostabilizing agent; rather, salt-induced enhancement of MV yields appeared to be due to intracellular events, e.g., augmented viral protein synthesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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