Investigation of the effect of ghrelin on bone fracture healing in rats.

Autor: Erener T; Department of Orthopaedics and Traumatology, Emirdağ State Hospital, Afyonkarahisar, Turkey., Ceritoğlu KU; Department of Orthopaedics and Traumatology, Çanakkale Onsekiz Mart University, Çanakkale, Turkey., Aktekin CN; Department of Orthopaedics and Traumatology, Yıldırım Beyazıt University, Ankara, Turkey., Dalgic AD; Department of Genetics and Bioengineering, Istanbul Bilgi University, Istanbul, Turkey., Keskin D; Department of Engineering Sciences, Middle East Technical University, Ankara, Turkey., Geneci F; Department of Anatomy, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey., Ocak M; Department of Anatomy, Faculty of Dentistry, Ankara University, Ankara, Turkey., Bilecenoğlu B; Department of Anatomy, Faculty of Medicine, Medipol University, Ankara, Turkey., Hücümenoğlu S; Department of Pathology, Ankara Training and Research Hospital, Health Sciences University, Ankara, Turkey., Çaydere M; Department of Pathology, Ankara Training and Research Hospital, Health Sciences University, Ankara, Turkey., Şeneş M; Department of Biochemistry, Ankara Training and Research Hospital, Health Sciences University, Ankara, Turkey., Sezgin Ö; Department of Biochemistry, Bakırköy Training and Research Hospital, Health Sciences University, İstanbul, Turkey.
Jazyk: angličtina
Zdroj: Clinical and experimental pharmacology & physiology [Clin Exp Pharmacol Physiol] 2021 Oct; Vol. 48 (10), pp. 1382-1390. Date of Electronic Publication: 2021 Jul 05.
DOI: 10.1111/1440-1681.13544
Abstrakt: Ghrelin is known to have effects on proliferation and differentiation of osteoblasts and improvement of bone mineral density in rats. However, no experimental research on ghrelin's effects on fracture healing has been reported. In this context, the effect of ghrelin on the union of femoral shaft fractures was examined in this study by evaluating whether ghrelin will directly contribute to fracture healing. Forty male Wistar-Albino rats were divided into two groups as control and experimental (ghrelin treated) and standard closed shaft fractures were created in the left femurs of all rats. Daily ghrelin injections were applied to the experimental groups and equal numbers of rats were killed after 14 and 28 days following fracture formation. Tissue samples were examined with radiological, biomechanical, biochemical and histological analyses. Densitometry study showed that bone mineral density was improved after 28 days of ghrelin treatment compared to control. On histological examination, at the end of the 14 and 28 days of recovery, significant union was observed in the ghrelin-treated group. The ghrelin-treated group had higher breaking strength and stiffness at the end of 28 days of recovery. Biochemically, ALP levels were found to be higher in the ghrelin-treated group at the end of 28 days of recovery. Results showed that ghrelin directly contributes to fracture healing and it is promising to consider the effect of ghrelin on fracture healing in human studies with pharmacological applications.
(© 2021 John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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