DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika.

Autor:	Raclariu-Manolică AC; Natural History Museum, University of Oslo, Oslo, Norway.; Stejarul Research Centre for Biological Sciences, National Institute of Research and Development for Biological Sciences, Piatra Neamt, Romania., Anmarkrud JA; Natural History Museum, University of Oslo, Oslo, Norway., Kierczak M; Researgene, Uppsala, Sweden., Rafati N; Researgene, Uppsala, Sweden., Thorbek BLG; Natural History Museum, University of Oslo, Oslo, Norway., Schrøder-Nielsen A; Natural History Museum, University of Oslo, Oslo, Norway., de Boer HJ; Natural History Museum, University of Oslo, Oslo, Norway.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2021 Jun 04; Vol. 12, pp. 665618. Date of Electronic Publication: 2021 Jun 04 (Print Publication: 2021).
DOI:	10.3389/fpls.2021.665618
Abstrakt:	Herbs and spices are some of the most vulnerable products in terms of fraud and adulteration in the food sector. Although standard analytical methods are accurate for quality control of specific lead or marker compounds, they cannot accurately assess the entire species composition of many marketed products. Complementary analytical approaches are thus often used for comprehensive screening of herbs and spices. In this study we evaluate DNA metabarcoding for the identification and authentication of 62 products, containing basil, oregano, and paprika collected from different retailers and importers in Norway. Our results show varying degrees of discrepancy between the constituent species and those listed on the product labels, despite high product authenticity. We suggest the false positives result from the sensitivity of DNA metabarcoding and filtering thresholds should be integrated into protocols to reduce false positives. Our results highlight how integrating DNA metabarcoding into the toolbox of analytical methods for quality control of fresh and/or processed plant-based food can improve product quality. Competing Interests: NR and MK are both founders of the company Researgene. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Raclariu-Manolică, Anmarkrud, Kierczak, Rafati, Thorbek, Schrøder-Nielsen and de Boer.)
Databáze:	MEDLINE
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