MiR-206 regulates the Th17/Treg ratio during osteoarthritis.

Autor: Ye X; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China., Lu Q; Department of Orthopedics, Hubei 672 Orthopaedics Hospital of Integrated Chinese & Western Medicine, Wuhan, 430079, Hubei, China., Yang A; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China. yangaofei_5013@163.com.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China. yangaofei_5013@163.com.; College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China. yangaofei_5013@163.com., Rao J; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China., Xie W; Department of Orthopedics, Hubei 672 Orthopaedics Hospital of Integrated Chinese & Western Medicine, Wuhan, 430079, Hubei, China., He C; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China., Wang W; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China., Li H; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China., Zhang Z; Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430061, Hubei, China.; Institute of Orthopedics, Hubei Province Academy of Traditional Chinese Medicine, No.4, Hua-Yuan-Shan, Yanzhi Road, Wuchang District, Wuhan, 430070, Hubei, China.
Jazyk: angličtina
Zdroj: Molecular medicine (Cambridge, Mass.) [Mol Med] 2021 Jun 19; Vol. 27 (1), pp. 64. Date of Electronic Publication: 2021 Jun 19.
DOI: 10.1186/s10020-021-00315-1
Abstrakt: Background: The present study aimed to determine the functional role of miR-206 in T helper 17 (Th17)/regulatory T (Treg) cell differentiation during the development of osteoarthritis (OA).
Methods: Patients with OA and healthy controls were recruited for investigating the association between miR-206 and Th17/Treg ratio. Transfection experiments were conducted in CD4 + T cells to verify the mechanism of miR-206 on the balance of Treg/Th17. OA model was constructed to detect the clinical score, histopathological changes and Treg/Th17 ratio. OA model was induced in rats to verify the effect of miR-206 inhibition on Th17/Treg immunoregulation.
Results: High expression of miR-206 was positively correlated with peripheral Th17/Treg imbalance in patients with OA. The interactions between miR-206 and the 3' untranslated regions (3'-UTR) of suppressor of cytokine signaling-3 (SOCS3) and fork head transcriptional factor 3 (Foxp3) were confirmed by luciferase reporter assays. MiR-206 disturbed the Th17/Treg balance by targeting SOCS3 and Foxp3. In vivo assay demonstrated that antagomiR directed against miR-206 restored Th17/Treg balance during the development of OA.
Conclusion: MiR-206 contributed to the progression of OA by modulating Th17/Treg imbalance, suggesting that miR-206 inhibition might be a promising therapeutic strategy for the treatment of OA.
Databáze: MEDLINE
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