A Light-Oxygen-Voltage Receptor Integrates Light and Temperature.

Autor: Dietler J; Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany., Schubert R; Biophysical Chemistry, Humboldt-University Berlin, 10115 Berlin, Germany., Krafft TGA; Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany., Meiler S; Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany., Kainrath S; Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton, Victoria 3800, Australia., Richter F; Biophysical Chemistry, Humboldt-University Berlin, 10115 Berlin, Germany., Schweimer K; Biopolymers, University of Bayreuth, 95447 Bayreuth, Germany; North-Bavarian NMR Center, University of Bayreuth, 95447 Bayreuth, Germany., Weyand M; Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany., Janovjak H; Australian Regenerative Medicine Institute (ARMI), Monash University, Clayton, Victoria 3800, Australia., Möglich A; Department of Biochemistry, University of Bayreuth, 95447 Bayreuth, Germany; Biophysical Chemistry, Humboldt-University Berlin, 10115 Berlin, Germany; Bayreuth Center for Biochemistry & Molecular Biology, University of Bayreuth, 95447 Bayreuth, Germany; North-Bavarian NMR Center, University of Bayreuth, 95447 Bayreuth, Germany. Electronic address: andreas.moeglich@uni-bayreuth.de.
Jazyk: angličtina
Zdroj: Journal of molecular biology [J Mol Biol] 2021 Jul 23; Vol. 433 (15), pp. 167107. Date of Electronic Publication: 2021 Jun 17.
DOI: 10.1016/j.jmb.2021.167107
Abstrakt: Sensory photoreceptors enable organisms to adjust their physiology, behavior, and development in response to light, generally with spatiotemporal acuity and reversibility. These traits underlie the use of photoreceptors as genetically encoded actuators to alter by light the state and properties of heterologous organisms. Subsumed as optogenetics, pertinent approaches enable regulating diverse cellular processes, not least gene expression. Here, we controlled the widely used Tet repressor by coupling to light-oxygen-voltage (LOV) modules that either homodimerize or dissociate under blue light. Repression could thus be elevated or relieved, and consequently protein expression was modulated by light. Strikingly, the homodimeric RsLOV module from Rhodobacter sphaeroides not only dissociated under light but intrinsically reacted to temperature. The limited light responses of wild-type RsLOV at 37 °C were enhanced in two variants that exhibited closely similar photochemistry and structure. One variant improved the weak homodimerization affinity of 40 µM by two-fold and thus also bestowed light sensitivity on a receptor tyrosine kinase. Certain photoreceptors, exemplified by RsLOV, can evidently moonlight as temperature sensors which immediately bears on their application in optogenetics and biotechnology. Properly accounted for, the temperature sensitivity can be leveraged for the construction of signal-responsive cellular circuits.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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