Preventing post-surgical cardiac adhesions with a catechol-functionalized oxime hydrogel.

Autor: Fujita M; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Policastro GM; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Burdick A; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Lam HT; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Ungerleider JL; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Braden RL; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA., Huang D; Department of Medicine, University of California, San Diego, San Diego, CA, USA., Osborn KG; Division of Comparative Pathology and Medicine, School of Medicine, University of California, San Diego, San Diego, CA, USA., Omens JH; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA.; Department of Medicine, University of California, San Diego, San Diego, CA, USA., Madani MM; Division of Cardiovascular and Thoracic Surgery, University of California, San Diego, San Diego, CA, USA., Christman KL; Department of Bioengineering, University of California, San Diego, San Diego, CA, USA. christman@eng.ucsd.edu.; Sanford Consortium for Regenerative Medicine, University of California, San Diego, San Diego, CA, USA. christman@eng.ucsd.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 Jun 18; Vol. 12 (1), pp. 3764. Date of Electronic Publication: 2021 Jun 18.
DOI: 10.1038/s41467-021-24104-w
Abstrakt: Post-surgical cardiac adhesions represent a significant problem during routine cardiothoracic procedures. This fibrous tissue can impair heart function and inhibit surgical access in reoperation procedures. Here, we propose a hydrogel barrier composed of oxime crosslinked poly(ethylene glycol) (PEG) with the inclusion of a catechol (Cat) group to improve retention on the heart for pericardial adhesion prevention. This three component system is comprised of aldehyde (Ald), aminooxy (AO), and Cat functionalized PEG mixed to form the final gel (Ald-AO-Cat). Ald-AO-Cat has favorable mechanical properties, degradation kinetics, and minimal swelling, as well as superior tissue retention compared to an initial Ald-AO gel formulation. We show that the material is cytocompatible, resists cell adhesion, and led to a reduction in the severity of adhesions in an in vivo rat model. We further show feasibility in a pilot porcine study. The Ald-AO-Cat hydrogel barrier may therefore serve as a promising solution for preventing post-surgical cardiac adhesions.
Databáze: MEDLINE
Externí odkaz: