Exposure to 2,4-D herbicide induces hepatotoxicity in zebrafish larvae.

Autor: Martins RX; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Vieira L; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Souza JACR; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Silva MGF; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Muniz MS; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Souza T; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Queiroga FR; Laboratory of Immunology and Pathology of Invertebrates (LABIPI), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Machado MRF; Laboratory of Biotechnology and Physiology of Fishes (LabFish), Federal University of Jataí, Jataí, Brazil., da Silva PM; Laboratory of Immunology and Pathology of Invertebrates (LABIPI), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil., Farias D; Laboratory for Risk Assessment of Novel Technologies (LabRisk), Department of Molecular Biology, Federal University of Paraiba, João Pessoa, Brazil. Electronic address: davi@dbm.ufpb.br.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2021 Oct; Vol. 248, pp. 109110. Date of Electronic Publication: 2021 Jun 16.
DOI: 10.1016/j.cbpc.2021.109110
Abstrakt: 2,4-Dichlorophenoxyacetic acid (2,4-D) herbicide is the main ingredient in over 1500 commercially available products such as Weedestroy® AM40 and DMA® 4 IVM. Although the liver has been identified as one of the organs that are affected by this herbicide, reports on its hepatotoxic effects available in the literature are restricted to rats. Thus, there is a gap in information on other organisms that may be vulnerable to 2,4-D exposure, such as fish. Therefore, the present work aimed to assess the hepatotoxic potential of 2,4-D in fish using zebrafish (Danio rerio) larvae as a model system. For this purpose, its acute toxicity to zebrafish embryos was assessed, as well as its sublethal effects (< LC 50 ) on the activity of enzymes related to oxidative (GST, CAT and GPX) and metabolic (LDH) stress and liver parameters (AST, ALT and ALP) after 48 h of exposure. Morphological analyses of the liver were also assessed in zebrafish larvae. As a result, 2,4-D reduced larvae survival (LC 50 15.010 mg/L in 96 h of exposure), induced malformations, altered the activity of LDH, GST and CAT enzymes and significantly increased the activity of all biomarkers for liver damage. Although no changes in the color or size of larval liver were observed, histopathological analysis revealed that treatment with 2,4-D caused severe changes in liver tissue, such as vacuolization of the cytosol, eccentric cell nucleus, loss of tissue architecture and cellular boundaries. Thus, the results showed that 2,4-D altered the enzymatic profile related to oxidative stress, and induces liver damage.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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