THE EFFECTS OF ETANERCEPT AND CABERGOLINE ON ENDOMETRIOTIC IMPLANTS, UTERUS AND OVARIES IN RAT ENDOMETRIOSIS MODEL.

Autor: Keleş CD; Department of Obstetrics & Gynecology, Milas Government Hospital, Muğla, Turkey., Vural B; Department of Obstetrics & Gynecology, Assisted Reproductive Technology Unit, Şişli Kolan International Hospital, İstanbul, Turkey. Electronic address: vuralbirol@yahoo.com.tr., Filiz S; Department of Histology & Embryology, Assisted Reprodoctive Technology Unit, Kocaeli University School of Medicine, Kocaeli, Turkey., Vural F; Department of Obstetrics & Gynecology, Haydarpaşa Numune Training and Research Hospital, Health Sciences University, Hamidiye Medical Faculty, Istanbul, Turkey., Gacar G; Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Kocaeli University School of Medicine, Kocaeli, Turkey., Eraldemir FC; Department of Medical Biochemistry, Kocaeli University School of Medicine, Kocaeli, Turkey., Kurnaz S; Department of Histology & Embryology, Kocaeli University School of Medicine, Kocaeli, Turkey.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2021 Aug; Vol. 146, pp. 103340. Date of Electronic Publication: 2021 May 29.
DOI: 10.1016/j.jri.2021.103340
Abstrakt: The pathophysiology of endometriosis is still unknown and treatment options remain controversial. Searches focus on angiogenesis, stem cells, immunologic and inflammatory factors. This study investigated the effects of etanercept and cabergoline on ovaries, ectopic, and eutopic endometrium in an endometriosis rat model. This randomized, placebo-controlled, blinded study included 50 rats, Co(control), Sh(Sham), Cb(cabergoline), E(etanercept), and E + Cb(etanercept + cabergoline) groups. After surgical induction of endometriosis, 2 nd operation was performed for endometriotic volume and AMH level. After 15 days of treatment: AMH level, flow cytometry, implant volume, histologic scores, immunohistochemical staining of ectopic, eutopic endometrium, and ovary were evaluated at 3 rd operation. All groups had significantly reduced volume, TNF-α, VEGF, and CD 146/PDGF-Rβ staining of endometriotic implants comparing to the Sh group (p < 0.05).TNF-α staining of eutopic endometrium in all treatment groups was similar to Sh and Co groups (p > 0.05). E and E + Cb groups significantly decreased TNF-α staining in the ovary comparing to Sh, Co, and Cb groups (p < 0.05). All treatment groups had significantly higher AFC compared to the Sh group. CD25 + Cells' median percentage was significantly increased in the E + Cb group compared to Co, Sh, Cb, and E group. E + Cb group had a significantly higher CD5 + Cells' level than the Co group (p = 0.035). In conclusion; Etanercept and/or Cabergoline decreased volume, TNF-α, VEGF, and CD 146/PDGF-Rβ staining of the ectopic endometrial implant. E and E + Cb treatment decreased TNF-α levels in the ovary. E + Cb also increased peripheral blood CD25 + & CD5 + Cell's.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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