Structural dynamics of the chromo-shadow domain and chromodomain of HP1 bound to histone H3K9 methylated peptide, as measured by site-directed spin-labeling EPR spectroscopy.

Autor: Suetake I; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan; Center for Twin Research, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan; Department of Nutritional Sciences, Graduate School of Nutritional Sciences, Nakamura Gakuen University, Fukuoka, 814-0198, Japan. Electronic address: hotsuetake@hotmail.com., Nakazawa S; Department of Chemistry and Molecular Materials Sciences, Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan., Sato K; Department of Chemistry and Molecular Materials Sciences, Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan., Mutoh R; Department of Applied Physics, Faculty of Science, Fukuoka University, Fukuoka, 814-0180, Japan., Mishima Y; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Kawakami T; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Takei T; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Watanabe M; Center for Twin Research, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan., Sakai N; Center for Twin Research, Graduate School of Medicine, Osaka University, Osaka, 565-0871, Japan., Fujiwara T; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Takui T; Department of Chemistry and Molecular Materials Sciences, Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan., Miyata M; Department of Biology, Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan., Shinohara A; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Hojo H; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan., Arata T; Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan; Department of Biology, Graduate School of Science, Osaka City University, Osaka, 558-8585, Japan. Electronic address: arata@osaka-cu.ac.jp.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Aug 27; Vol. 567, pp. 42-48. Date of Electronic Publication: 2021 Jun 15.
DOI: 10.1016/j.bbrc.2021.06.010
Abstrakt: The structural dynamics of the chromo-shadow domain (CSD) and chromodomain (CD) of human HP1 proteins essential for heterochromatin formation were investigated at the nanosecond and nanometer scales by site-directed spin labeling electron paramagnetic resonance and pulsed double resonance spectroscopy. Distance measurements showed that the spin-labeled CSD of human HP1α and HP1γ tightly dimerizes. Unlike CD-CD interaction observed in fission yeast HP1 in an inactivated state (Canzio et al., 2013), the two CDs of HP1α and HP1γ were spatially separated from each other, dynamically mobile, and ready for a Brownian search for H3K9-tri-methyl(me3) on histones. Complex formation of the CD with H3K9me3 slowed dynamics of the domain due to a decreased diffusion constant. CSD mobility was significantly (∼1.3-fold) lower in full-length HP1α than in HP1γ, suggesting that the immobilized conformation of human HP1α shows an auto-inactivated state. Differential properties of HP1α and HP1γ to form the inactive conformation could be relevant to its physiological role in the heterochromatin formation in a cell.
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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