High-resolution melting curve FRET-PCR rapidly identifies SARS-CoV-2 mutations.

Autor: Barua S; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA., Hoque M; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA., Kelly PJ; Ross University School of Veterinary Medicine, Basseterre, Saint George Basseterre, Saint Kitts and Nevis., Bai J; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Hanzlicek G; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Noll L; Kansas State Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, USA., Walz H; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.; Thompson Bishop Sparks State Diagnostic Laboratory, Auburn, Alabama, USA., Johnson C; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA., Kyriakis C; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA., Wang C; College of Veterinary Medicine, Auburn University, Auburn, Alabama, USA.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2021 Sep; Vol. 93 (9), pp. 5588-5593. Date of Electronic Publication: 2021 Jun 22.
DOI: 10.1002/jmv.27139
Abstrakt: Reverse transcription fluorescence resonance energy transfer-polymerase chain reaction (FRET-PCRs) were designed against the two most common mutations in severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (A23403G in the spike protein; C14408T in the RNA-dependent RNA polymerase). Based on high-resolution melting curve analysis, the reverse transcription (RT) FRET-PCRs identified the mutations in american type culture collection control viruses, and feline and human clinical samples. All major makes of PCR machines can perform melting curve analysis and thus further specifically designed FRET-PCRs could enable active surveillance for mutations and variants in countries where genome sequencing is not readily available.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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