Mapping the distribution and risk factors of Anaplasmataceae in wild and domestic canines in Chile and their association with Rhipicephalus sanguineus species complex lineages.

Autor: S DC; Conservation Medicine, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile. Electronic address: sophidica@hotmail.com., A C; Facultad de Ciencias de la Vida, Universidad Andres Bello, República 440, Santiago, Chile; Department of Animal Health. NEIKER-Basque Institute for Agricultural Research and Development. Basque Research and Technology Alliance (BRTA). Parque Científico y Tecnológico de Bizkaia, P812, 48160 Derio, Spain., C UC; Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santa Rosa 11735, La Pintana, Santiago, Chile., E HH; Conservation and Research Department, Parque Zoológico Buin Zoo, Panamericana Sur Km 32, Buin, Chile., V G; Centro di Referenza Nazionale per Anaplasma, Babesia, Rickettsia, e Theileria, Istituto zooprofilattico sperimentale della Sicilia, via Gino Marinuzzi, 3, Palermo, Sicilia, Italy., I S; Facultad de Ciencias de la Vida, Universidad Andres Bello, República 440, Santiago, Chile., N SP; Unidad de Rehabilitación de Fauna Silvestre, Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile., D PM; Parque Safari Chile, Ruta H-30, Km 5, Camino A Doñihue S/N, Rancagua, O'Higgins, Chile., D GA; Departamento de Ciencias Pecuarias, Facultad de Medicina Veterinaria, 71 Universidad de Concepción, Chillán, Chile., C N; Departamento de Ciencias Biológicas y Biodiversidad, Universidad de Los Lagos, Av. Fuchslocher 1305, Osorno, Chile; Instituto de Ecología y Biodiversidad, Santiago, Chile., J V; Departamento de Ecosistemas y Medio Ambiente, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Av. Vicuña Mackenna 4860, Santiago, Chile., G AJ; Instituto de Medicina Preventiva Veterinaria y Programa de Investigación Aplicada en Fauna Silvestre, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile., D V; Centro di Referenza Nazionale per Anaplasma, Babesia, Rickettsia, e Theileria, Istituto zooprofilattico sperimentale della Sicilia, via Gino Marinuzzi, 3, Palermo, Sicilia, Italy., J M; Facultad de Ciencias de la Vida, Universidad Andres Bello, República 440, Santiago, Chile; Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013 Zaragoza, Spain; Fundación ARAID, Avda. de Ranillas, 50018 Zaragoza, Spain.
Jazyk: angličtina
Zdroj: Ticks and tick-borne diseases [Ticks Tick Borne Dis] 2021 Sep; Vol. 12 (5), pp. 101752. Date of Electronic Publication: 2021 Jun 04.
DOI: 10.1016/j.ttbdis.2021.101752
Abstrakt: Anaplasma platys and Ehrlichia canis are members of the Anaplasmataceae family that cause disease in dogs and are mainly transmitted by Rhipicephalus sanguineus species group ticks. We performed a cross-sectional study on these pathogens across six bioclimatic regions of Chile, including 719 free-ranging rural dogs, 132 Andean foxes (Lycalopex culpaeus), and 82 South American gray foxes (Lycalopex griseus). Dog and fox blood samples were first screened for DNA of Anaplasmataceae followed by two Ehrlichia-specific protocols. Antibodies against Anaplasma sp. and E. canis were assessed by immunofluorescence in dogs. Ectoparasites were collected and identified, with the determination of the lineages of the Rhipicephalus sanguineus species group by molecular and phylogenetic analyses. Finally, potential risk factors for infection were investigated across the different bioclimatic regions and host species. All DNA amplicons obtained from the screening protocol corresponded to Anaplasma platys. The occurrence of both A. platys DNA and antibodies was confirmed in all six bioclimatic regions, except for regions at high altitude and/or without either R. sanguineus species group lineage present. Dogs infested with R. sanguineus ticks were significantly more prone to be infected and exposed to Anaplasma spp. Prevalence of DNA was significantly higher in juvenile (19%) than in adult dogs (9%), whereas the opposite was found for seroprevalence (19% versus 35%, respectively). Overall prevalence of A. platys DNA was higher in dogs (11%) than in foxes (4%), probably owing to markedly lower tick infestations in the foxes. Ehrlichia canis DNA was not detected in any sample, and antibodies against this pathogen were detected only in four dogs, in areas with both R. sanguineus lineages present. Free-ranging dogs in Chile could be favoring the maintenance of A. platys in all areas suitable for its tick vector. Although apparently infrequent, spillovers from dogs to foxes may be taking place and should be considered in management plans in Chile.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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