Vatairea guianensis lectin stimulates changes in gene expression and release of TNF-α from rat peritoneal macrophages via glycoconjugate binding.

Autor: Marques GFO; Instituto de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Brazil., Pires AF; Instituto de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Brazil., Osterne VJS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.; Departamento de Nutrição, Universidade Estadual do Ceará, Fortaleza, Brazil., Pinto-Junior VR; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.; Departamento de Física, Universidade Federal do Ceará, Fortaleza, Brazil., Silva IB; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil., Martins MGQ; Instituto Superior de Teologia Aplicada - INTA, Sobral, Brazil., Oliveira MV; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil., Gomes AM; Instituto de Bioquímica Médica e Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Souza LAG; Instituto Nacional de Pesquisas da Amazônia - INPA, Manaus, Brazil., Pavão MSG; Instituto de Bioquímica Médica e Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Cavada BS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil., Assreuy AMS; Instituto de Ciências Biomédicas, Universidade Estadual do Ceará, Fortaleza, Brazil., Nascimento KS; Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Brazil.
Jazyk: angličtina
Zdroj: Journal of molecular recognition : JMR [J Mol Recognit] 2021 Oct; Vol. 34 (10), pp. e2922. Date of Electronic Publication: 2021 Jun 16.
DOI: 10.1002/jmr.2922
Abstrakt: Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose-dependent gene expression and release of TNF-α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF-α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P < .05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin-induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF-α gene expression (RT-PCR) and release from peritoneal macrophages. Thus, upon lectin-glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF-α and TLR and/or TNFR receptor pathways.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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