Low Carbon Footprint Recycling of Post-Consumer PET Plastic with a Metagenomic Polyester Hydrolase.

Autor: Sonnendecker C; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany., Oeser J; Department of Microbiology and Bioprocess Technology Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany., Richter PK; Institute of Bioanalytical Chemistry, Centre for Biotechnology and Biomedicine, Leipzig University, 04103, Leipzig, Germany., Hille P; Department of Microbiology and Bioprocess Technology Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany., Zhao Z; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany., Fischer C; Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Institut für Ressourcenökologie Abteilung Reaktiver Transport, D-04318, Leipzig, Germany., Lippold H; Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Institut für Ressourcenökologie Abteilung Reaktiver Transport, D-04318, Leipzig, Germany., Blázquez-Sánchez P; Institute for Biological and Medical Engineering Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, 7820436, Chile.; ANID-Millennium Science Initiative Program, Millennium Institute for Integrative Biology (iBio), Santiago, 8331150, Chile., Engelberger F; Institute for Biological and Medical Engineering Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, 7820436, Chile.; ANID-Millennium Science Initiative Program, Millennium Institute for Integrative Biology (iBio), Santiago, 8331150, Chile., Ramírez-Sarmiento CA; Institute for Biological and Medical Engineering Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago, 7820436, Chile.; ANID-Millennium Science Initiative Program, Millennium Institute for Integrative Biology (iBio), Santiago, 8331150, Chile., Oeser T; Department of Microbiology and Bioprocess Technology Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany., Lihanova Y; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany., Frank R; Centre for Biotechnology and Biomedicine Molecular Biological-Biochemical Processing Technology, Leipzig University, 04103, Leipzig, Germany., Jahnke HG; Centre for Biotechnology and Biomedicine Molecular Biological-Biochemical Processing Technology, Leipzig University, 04103, Leipzig, Germany., Billig S; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany., Abel B; Leibniz Institute of Surface Engineering (IOM), Wilhelm-Ostwald-Institute of Physical and Theoretical Chemistry, 04103, Leipzig, Germany., Sträter N; Institute of Bioanalytical Chemistry, Centre for Biotechnology and Biomedicine, Leipzig University, 04103, Leipzig, Germany., Matysik J; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany., Zimmermann W; Institute of Analytical Chemistry, Leipzig University, 04103, Leipzig, Germany.; Department of Microbiology and Bioprocess Technology Institute of Biochemistry, Leipzig University, 04103, Leipzig, Germany.
Jazyk: angličtina
Zdroj: ChemSusChem [ChemSusChem] 2022 May 06; Vol. 15 (9), pp. e202101062. Date of Electronic Publication: 2022 Feb 10.
DOI: 10.1002/cssc.202101062
Abstrakt: Earth is flooded with plastics and the need for sustainable recycling strategies for polymers has become increasingly urgent. Enzyme-based hydrolysis of post-consumer plastic is an emerging strategy for closed-loop recycling of polyethylene terephthalate (PET). The polyester hydrolase PHL7, isolated from a compost metagenome, completely hydrolyzes amorphous PET films, releasing 91 mg of terephthalic acid per hour and mg of enzyme. Vertical scanning interferometry shows degradation rates of the PET film of 6.8 μm h -1 . Structural analysis indicates the importance of leucine at position 210 for the extraordinarily high PET-hydrolyzing activity of PHL7. Within 24 h, 0.6 mg enzyme  g PET -1 completely degrades post-consumer thermoform PET packaging in an aqueous buffer at 70 °C without any energy-intensive pretreatments. Terephthalic acid recovered from the enzymatic hydrolysate is then used to synthesize virgin PET, demonstrating the potential of polyester hydrolases as catalysts in sustainable PET recycling processes with a low carbon footprint.
(© 2021 The Authors. ChemSusChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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