| Autor: |
Tan C; Department of Epidemiology & Health Statistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China.; Department of Epidemiology & Health Statistics, School of Public Health, Guilin Medical University, Guilin, Guangxi, 541100, PR China., Zeng X; Department of Occupational & Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China.; Department of Occupational & Environmental Health, School of Public Health, Guilin Medical University, Guilin, Guangxi, 541100, PR China., Mo M; Department of Epidemiology & Health Statistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Ma X; Department of Epidemiology & Health Statistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Liang Q; Department of Epidemiology & Health Statistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Liang W; Department of Social Medicine, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Huang W; Department of Occupational & Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Wang K; Department of Occupational & Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Yang L; Department of Occupational & Environmental Health, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China., Qiu X; Department of Epidemiology & Health Statistics, School of Public Health, Guangxi Medical University, Nanning, Guangxi, 530021, PR China. |
| Abstrakt: |
Aim: To explore the expression profiles of long noncoding RNAs (lncRNAs) and identify novel lncRNAs as biomarkers for early diagnosis and therapy of hepatocellular carcinoma (HCC). Materials & methods: Expression profiles of lncRNAs and mRNAs in five paired HCC and adjacent normal tissues were obtained by RNA sequencing. Eight lncRNAs, including two novel liver-specific lncRNAs (NONHSAT059247.2 and NONHSAT013897.2), were validated in another 74 pairs of HCC and adjacent normal tissues by quantitative reverse transcription PCR. Results: The results of quantitative reverse transcription PCR showed that NONHSAT252133.1, NONHSAT112116.2 and NONHSAT242657.1 were significantly upregulated in HCC tissues, whereas NONHSAT169790.1, NONHSAT059247.2 and NONHSAT013897.2 were significantly downregulated. Two liver-specific lncRNAs demonstrated excellent diagnostic performance: NONHSAT059247.2 (area under the curve = 0.941, 95% CI: 0.902-0.979, p < 0.0001), NONHSAT013897.2 (area under the curve = 0.944, 95% CI: 0.906-0.983, p < 0.0001). Conclusion: The liver-specific lncRNAs NONHSAT059247.2 and NONHSAT013897.2, may provide new biomarkers for the future study on diagnosis, therapy and mechanisms of HCC. |
