WRKY46 promotes ammonium tolerance in Arabidopsis by repressing NUDX9 and indole-3-acetic acid-conjugating genes and by inhibiting ammonium efflux in the root elongation zone.

Autor: Di DW; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China., Sun L; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, Jiangsu, 210095, China., Wang M; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China., Wu J; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.; Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, 210014, China., Kronzucker HJ; School of BioSciences, The University of Melbourne, Parkville, Vic., 3010, Australia.; Faculty of Land and Food Systems, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada., Fang S; National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100049, China., Chu J; National Centre for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100049, China., Shi W; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China., Li G; State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2021 Oct; Vol. 232 (1), pp. 190-207. Date of Electronic Publication: 2021 Jul 16.
DOI: 10.1111/nph.17554
Abstrakt: Ammonium (NH 4 + ) is toxic to root growth in most plants, even at moderate concentrations. Transcriptional regulation is one of the most important mechanisms in the response of plants to NH 4 + toxicity, but the nature of the involvement of transcription factors (TFs) in this regulation remains unclear. Here, RNA-seq analysis was performed on Arabidopsis roots to screen for ammonium-responsive TFs. WRKY46, the member of the WRKY transcription factor family most responsive to NH 4 + , was selected. We defined the role of WRKY46 using mutation and overexpression assays, and characterized the regulation of NUDX9 and indole-3-acetic acid (IAA)-conjugating genes by WRKY46 via yeast one-hybrid and electrophoretic mobility shift assays and chromatin immunoprecipitation-quantitative real-time polymerase chain reaction (ChIP-qPCR). Knockout of WRKY46 increased, while overexpression of WRKY46 decreased, NH 4 + -suppression of the primary root. WRKY46 is shown to directly bind to the promoters of the NUDX9 and IAA-conjugating genes (GH3.1, GH3.6, UGT75D1, UGT84B2) and to inhibit their transcription, thus positively regulating free IAA content and stabilizing protein N-glycosylation, leading to an inhibition of NH 4 + efflux in the root elongation zone (EZ). We identify TF involvement in the regulation of NH 4 + efflux in the EZ, and show that WRKY46 inhibits NH 4 + efflux by negative regulation of NUDX9 and IAA-conjugating genes.
(© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation.)
Databáze: MEDLINE
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