Histopathological Parameters of Canine Bone Marrow in Cell-Block Preparations.

Autor: Varjão NM; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil., Araújo IBO; Department of Pathology and Forensic Medicine, Faculty of Medicine, Federal University of Bahia, Rua Salvador, BA, Brazil., Hlavac N; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil., Nunes TL; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil., Varjão BM; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil., de Pinho FA; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil., Barrouin-Melo SM; Laboratory of Veterinary Infectious Diseases, Teaching Hospital of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine, Federal University of Bahia, Salvador, BA, Brazil. Electronic address: barrouin@ufba.br.
Jazyk: angličtina
Zdroj: Topics in companion animal medicine [Top Companion Anim Med] 2021 Nov; Vol. 45, pp. 100552. Date of Electronic Publication: 2021 Jun 12.
DOI: 10.1016/j.tcam.2021.100552
Abstrakt: Studies using the cell-block technique for bone marrow (BM) analysis are limited in the veterinary literature. This work assessed whether the histopathological analysis of canine BM was feasible using cell-block cytoinclusions prepared from fine-needle sternal aspirate samples. Eight clinically healthy young-to-middle-aged dogs underwent both fine-needle sternal aspiration for BM cell-block (BM-Cb) processing and iliac-crest BM core biopsy (BM-B). Histopathologic parameters were compared between the 2 methods. There were no statistically significant histopathological differences between hematopoietic tissue areas (P =  .6294) in the BM-Cb and BM-B sections, and they had similar microscopic characteristics and microarchitecture. Cellularity and reticulin-fiber staining were equivalent in the BM-Cb and BM-B preparations in 87.5% (7/8) and 100% (8/8) of the sections, respectively. However, the quantitative results of the megakaryocytic series differed between BM-Cb and BM-B in 37.5% (3/8) of the sections, and the myeloid:erythroid (M:E) ratios differed between the 2 methods in 25% (2/8). These preliminary data indicate that cell-block preparations made from sternal fine-needle aspiration samples warrant continued evaluation in a larger number of animals, including those with various diseases affecting the bone marrow.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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