Type 1 Innate Lymphoid Cell and Natural Killer Cells Are Sources of Interferon-γ and Other Inflammatory Cytokines Associated With Distinct Clinical Presentation in Early Dengue Infection.

Autor:	Quintino-de-Carvalho IL; Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Gonçalves-Pereira MH; Departamento de Imunologia e Bioquímica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Faria Ramos M; Departamento de Imunologia e Bioquímica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., de Aguiar Milhim BHG; Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil., Da Costa ÚL; Secretaria Municipal de Saúde, Belo Horizonte, Minas Gerais, Brazil., Santos ÉG; Secretaria Municipal de Saúde, Belo Horizonte, Minas Gerais, Brazil., Nogueira ML; Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil., Da Costa Santiago H; Departamento de Imunologia e Bioquímica, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2022 Jan 05; Vol. 225 (1), pp. 84-93.
DOI:	10.1093/infdis/jiab312
Abstrakt:	Background: Increased levels of inflammatory cytokines are associated with severe dengue evolution, but the source of such hypercytokinemia is elusive. We investigated the contribution of innate lymphocytes, innate lymphoid cells (ILCs), and natural killer (NK) cells in cytokine production in early dengue infection. Methods: Peripheral blood mononuclear cells of individuals with dengue without warning signs (DWS-) and dengue with warning signs and severe dengue (SD) presentation combined (DWS+) were obtained between 2 and 7 days since fever onset and submitted to flow cytometry without specific antigen stimulation to evaluate cytokines in ILC and NK cell subpopulations. Results: ILCs and NK cells were found to be important sources of cytokines during dengue. ILCs of the DWS+/SD group displayed higher production of interferon gamma (IFN-γ) and interleukin (IL) 4/IL-13 when compared to DWS- individuals. On the other hand, NK Eomes+ cells of DWS- patients displayed higher IFN-γ production levels compared with the DWS+/SD group. Interestingly, when NK cells were identified by CD56 expression, DWS+/SD displayed higher frequency of IL-17 production compared with the DWS- group. Conclusions: These results indicate that ILCs and NK cells are important sources of inflammatory cytokines during acute dengue infection and display distinct profiles associated with different clinical forms. (© The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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