A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens.

Autor:	Wang X; Global Health Research Center, Duke Kunshan University, Kunshan, China.; School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia., Xiu L; Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA.; Duke Global Health Institute, Duke University, Durham, NC, USA.; NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China., Binder RA; Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA.; Duke Global Health Institute, Duke University, Durham, NC, USA., Toh TH; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia.; Clinical Research Center, Sibu Hospital, Ministry of Health Malaysia, Sibu, Sarawak, Malaysia., Lee JS; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia.; Clinical Research Center, Sibu Hospital, Ministry of Health Malaysia, Sibu, Sarawak, Malaysia., Ting J; Faculty of Medicine, SEGi University, Kota Damansara, Selangor, Malaysia., Than ST; Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore., Qi W; Global Health Research Center, Duke Kunshan University, Kunshan, China., Coleman KK; Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore., Perera D; Institute of Health and Community Medicine, Universiti Malaysia Sarawak, Kota Samarahan, Sarawak, Malaysia., Ma M; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100,071, China., Gray GC; Global Health Research Center, Duke Kunshan University, Kunshan, China.; Division of Infectious Diseases, Duke University School of Medicine, Durham, NC, USA.; Duke Global Health Institute, Duke University, Durham, NC, USA.; Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore.
Jazyk:	angličtina
Zdroj:	One health (Amsterdam, Netherlands) [One Health] 2021 Dec; Vol. 13, pp. 100274. Date of Electronic Publication: 2021 Jun 06.
DOI:	10.1016/j.onehlt.2021.100274
Abstrakt:	We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations. Competing Interests: None. (© 2021 The Authors.)
Databáze:	MEDLINE
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