Chemical synthesis and immunological evaluation of entirely carbohydrate conjugate Globo H-PS A1.

Autor:	Ghosh S; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Trabbic KR; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Shi M; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Nishat S; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Eradi P; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Kleski KA; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu., Andreana PR; The University of Toledo, Department of Chemistry and Biochemistry 2801 West Bancroft Street Toledo Ohio USA 43606 peter.andreana@utoledo.edu.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2020 Oct 19; Vol. 11 (48), pp. 13052-13059. Date of Electronic Publication: 2020 Oct 19.
DOI:	10.1039/d0sc04595k
Abstrakt:	An anticancer, entirely carbohydrate conjugate, Globo H-polysaccharide A1 (Globo H-PS A1), was chemically prepared and immunologically evaluated in C57BL/6 mice. Tumor associated carbohydrate antigen Globo H hexasaccharide was synthesized in an overall 7.8% yield employing a convergent [3 + 3] strategy that revealed an anomeric aminooxy group used for conjugation to oxidized PS A1 via an oxime linkage. Globo H-PS A1, formulated with adjuvants monophosphoryl lipid A and TiterMax® Gold. After immunization an antigen specific immune response was observed in ELISA with anti-Globo H IgG/IgM antibodies. Specificity of the corresponding antibodies was determined by FACS showing cell surface binding to Globo H-positive cancer cell lines MCF-7 and OVCAR-5. The anti-Globo H antibodies also exhibited complement-dependent cellular cytotoxicity against MCF-7 and OVCAR-5 cells. Competing Interests: The authors declare no conflict of interest. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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