Selective glucose sensing in complex media using a biomimetic receptor.

Autor:	Tromans RA; School of Chemistry, University of Bristol Cantock's Close Bristol BS8 1TS UK Anthony.Davis@bristol.ac.uk., Samanta SK; School of Chemistry, University of Bristol Cantock's Close Bristol BS8 1TS UK Anthony.Davis@bristol.ac.uk., Chapman AM; Carbometrics Ltd., Unit DX St Philips Central, Albert Road Bristol BS2 0XJ UK., Davis AP; School of Chemistry, University of Bristol Cantock's Close Bristol BS8 1TS UK Anthony.Davis@bristol.ac.uk.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2020 Feb 25; Vol. 11 (12), pp. 3223-3227. Date of Electronic Publication: 2020 Feb 25.
DOI:	10.1039/c9sc05406e
Abstrakt:	Glucose is a key biomedical analyte, especially relevant to the management of diabetes. Current methods for glucose determination rely on the enzyme glucose oxidase, requiring specialist instrumentation and suffering from redox-active interferents. In a new approach, a powerful and highly selective achiral glucose receptor is mixed with a sample, l-glucose is added, and the induced CD spectrum is measured. The CD signal results from competition between the enantiomers, and is used to determine the d-glucose content. The involvement of l-glucose doubles the signal range from the CD spectrometer and allows sensitivity to be adjusted over a wide dynamic range. It also negates medium effects, which must be equal for both enantiomers. The method has been demonstrated with human serum, pre-filtered to remove proteins, giving results which closely match the standard biochemical procedures, as well as a cell culture medium and a beer sample containing high (70 mM) and low (0.4 mM) glucose concentrations respectively. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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