Effects of Dietary Crude Glycerin Concentration on Testicular Morphology and Oxidative Stress Markers and on Plasma Testosterone Concentrations.

Autor: Venâncio AKLP; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil., Dias FCR; Department of Structural Biology, Universidade Federal do Triângulo Mineiro, Uberaba, , Brazil., Nascimento AS; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil., Ramos FF; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil., Oliveira F; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil., Torres SM; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil., Carvalho FFR; Department of Zootechnics, Universidade Federal Rural de Pernambuco, Recife, Brazil., da Silva Júnior VA; Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco, Recife, , Brazil. Electronic address: vajunior@dmv.ufrpe.br.
Jazyk: angličtina
Zdroj: Journal of comparative pathology [J Comp Pathol] 2021 May; Vol. 185, pp. 72-81. Date of Electronic Publication: 2021 May 23.
DOI: 10.1016/j.jcpa.2021.04.002
Abstrakt: We evaluated the effects of feeding 6%, 12% or 18% crude glycerin, containing 80.5% glycerol, on testicular histomorphometry and markers of oxidative stress and on plasma testosterone concentrations in lambs. Body weight, testicular biometric measurements, gonadosomatic index and net weight of the testicles were higher for the treated groups (P <0.05) compared with a control group that did not receive dietary glycerin. The mean total length of seminiferous tubules was higher in the 6% group (P <0.05), while the mean total tubular and seminiferous epithelium volumes increased in all treated groups (P <0.05). The volume of Leydig cells increased in the 12% group, while their number per gram of testicle decreased (P <0.05). There was a decrease in mean nuclear diameter and mean volume of Leydig cells, and an increase in the mean number of these cells per gram of testicle, in the 18% group (P <0.05). Plasma testosterone concentrations were unaffected. There was desquamation of seminiferous epithelium and vacuolation of Sertoli cells in the treated groups. Variable degrees of spermatocyte necrosis and the presence of giant cells were seen in all groups and there was intense vacuolation of Sertoli cells in the 12% and 18% groups. Superoxide dismutase and catalase production increased most in the 12% and 18% groups (P <0.05), while glutathione production was higher in the 18% group (P <0.05). Mean nitric oxide concentration decreased in all treated groups (P <0.05), while malondialdehyde production was higher in the 18% group than in the control and 6% groups (P <0.05). We conclude that the inclusion of 6% glycerin in the diet of lambs results in changes in testicular morphology that have been previously associated with improved reproductive function, but without evidence of oxidative stress.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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