Isolation and characterization of two highly insecticidal, endophytic strains of Bacillus thuringiensis.

Autor:	García-Suárez R; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500 Irapuato, Gto., Mexico., Verduzco-Rosas LA; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500 Irapuato, Gto., Mexico., Ibarra JE; Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 629, 36500 Irapuato, Gto., Mexico.
Jazyk:	angličtina
Zdroj:	FEMS microbiology ecology [FEMS Microbiol Ecol] 2021 Jun 18; Vol. 97 (7).
DOI:	10.1093/femsec/fiab080
Abstrakt:	Recent discovery of endophytic strains of Bacillus thuringiensis significantly improves the knowledge on its ecology. It also may be a new source for the isolation of insecticidal strains. This report shows the characterization of two endophytic, highly insecticidal strains of B. thuringiensis. Strains LBIT-1250L and LBIT-1251P were isolated from lavender and Poinsettia sap, respectively. Their parasporal crystals were very similar in morphology to those shown by serotypes israelensis and kurstaki, respectively. Bioassays on Aedes aegypti fourth instar larvae and on Manduca sexta first instar larvae, respectively, showed significantly higher levels of toxicity than those of their standard counterparts, IPS-82 (israelensis) and HD-1 (kurstaki) strains, respectively. Characterization of both strains included the sequencing of flagellin (hag) gene, plasmid and Bc Rep-PCR patterns and crystal protein content. All four characterization features indicated that LBIT1250L is highly related to the IPS-82 standard (serotype H-14: israelensis); while the LBIT-1251P was highly related to the HD-1 standard (serotype H-3a3b3c kurstaki). These results indicate that endophytic strains of B. thuringiensis may be a new source of potential insecticidal strains and opens more in-depth studies about the role of this bacterium in such a specialized habitat. (© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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