| Autor: |
Bram Ednersson S; Department of Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden.; Institute of Biomedicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Stern M; Department of Medicine, Section of Hematology, South Älvsborg Hospital, Borås, Sweden.; Institute of Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Fagman H; Department of Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden.; Institute of Biomedicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Nilsson-Ehle H; Institute of Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.; Section of Hematology and Coagulation, Sahlgrenska University Hospital, Gothenburg, Sweden., Hasselblom S; Department of Research, Development & Education, Region Halland, Halmstad, Sweden., Thorsell A; Proteomics Core Facility, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden., Andersson PO; Department of Medicine, Section of Hematology, South Älvsborg Hospital, Borås, Sweden.; Institute of Medicine, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden. |
| Abstrakt: |
The complexity of the activated B-cell like (ABC) diffuse large B-cell lymphoma (DLBCL) subtype is probably not only explained by genetic alterations and methods to measure global protein expression could bring new knowledge regarding the pathophysiology. We used quantitative proteomics to analyze the global protein expression of formalin-fixed paraffin-embedded (FFPE) tumor tissues from 202 DLBCL patients. We identified 6430 proteins and 498 were significantly regulated between the germinal center B-cell like (GCB) and non-GCB groups. A number of proteins previously not described to be upregulated in non-GCB or ABC DLBCL was found, e.g. CD64, CD85A, guanylate-binding protein 1 (GBP1), interferon-induced proteins with tetratricopeptide repeat (IFIT)2, and mixed lineage kinase domain-like protein (MLKL) and immunohistochemical staining showed higher expression of GBP1 and MLKL. A cluster analysis revealed that the most prominent cluster contained proteins involved in the tumor microenvironment and regulation of the immune system. Our data suggest that the therapeutic focus should be expanded toward the tumor microenvironment in non-GCB/ABC subtype patients. |
