Microtubule reorganization during female meiosis in C. elegans .

Autor:	Lantzsch I; Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany., Yu CH; Department of Electrical and Computer Engineering, University of California, Santa Barbara, Santa Barbara, United States., Chen YZ; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, United States.; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, United States., Zimyanin V; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, United States.; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, United States., Yazdkhasti H; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, United States.; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, United States., Lindow N; Zuse Institute Berlin, Berlin, Germany., Szentgyoergyi E; Experimental Center, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany., Pani AM; Department of Biology, University of Virginia, Charlottesville, United States.; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, United States., Prohaska S; Zuse Institute Berlin, Berlin, Germany., Srayko M; Department of Biological Sciences, University of Alberta, Edmonton, Canada., Fürthauer S; Center for Computational Biology, Flatiron Institute, New York, United States., Redemann S; Center for Membrane and Cell Physiology, University of Virginia School of Medicine, Charlottesville, United States.; Department of Molecular Physiology and Biological Physics, University of Virginia, School of Medicine, Charlottesville, United States.; Department of Cell Biology, University of Virginia School of Medicine, Charlottesville, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2021 Jun 11; Vol. 10. Date of Electronic Publication: 2021 Jun 11.
DOI:	10.7554/eLife.58903
Abstrakt:	Most female meiotic spindles undergo striking morphological changes while transitioning from metaphase to anaphase. The ultra-structure of meiotic spindles, and how changes to this structure correlate with such dramatic spindle rearrangements remains largely unknown. To address this, we applied light microscopy, large-scale electron tomography and mathematical modeling of female meiotic Caenorhabditis elegans spindles. Combining these approaches, we find that meiotic spindles are dynamic arrays of short microtubules that turn over within seconds. The results show that the metaphase to anaphase transition correlates with an increase in microtubule numbers and a decrease in their average length. Detailed analysis of the tomographic data revealed that the microtubule length changes significantly during the metaphase-to-anaphase transition. This effect is most pronounced for microtubules located within 150 nm of the chromosome surface. To understand the mechanisms that drive this transition, we developed a mathematical model for the microtubule length distribution that considers microtubule growth, catastrophe, and severing. Using Bayesian inference to compare model predictions and data, we find that microtubule turn-over is the major driver of the spindle reorganizations. Our data suggest that in metaphase only a minor fraction of microtubules, those closest to the chromosomes, are severed. The large majority of microtubules, which are not in close contact with chromosomes, do not undergo severing. Instead, their length distribution is fully explained by growth and catastrophe. This suggests that the most prominent drivers of spindle rearrangements are changes in nucleation and catastrophe rate. In addition, we provide evidence that microtubule severing is dependent on katanin. Competing Interests: IL, CY, YC, VZ, HY, NL, ES, AP, SP, MS, SF, SR No competing interests declared (© 2021, Lantzsch et al.)
Databáze:	MEDLINE
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