Characterization of carbapenem-resistant gram-negative bacterial isolates from Nigeria by whole genome sequencing.

Autor: Tickler IA; Cepheid, Sunnyvale, CA, USA. Electronic address: isabella.tickler@cepheid.com., Shettima SA; Federal Medical Centre, Yola, Nigeria., Dela Cruz CM; Cepheid, Sunnyvale, CA, USA., Le VM; Cepheid, Sunnyvale, CA, USA., Dewell S; Cepheid, Sunnyvale, CA, USA., Sumner J; Cepheid, Sunnyvale, CA, USA., Tenover FC; Cepheid, Sunnyvale, CA, USA.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2021 Sep; Vol. 101 (1), pp. 115422. Date of Electronic Publication: 2021 May 07.
DOI: 10.1016/j.diagmicrobio.2021.115422
Abstrakt: This study characterized the mechanisms of carbapenem resistance in gram-negative bacteria isolated from patients in Yola, Nigeria. Whole genome sequencing (WGS) was performed on 66 isolates previously identified phenotypically as carbapenem-non-susceptible. The patterns of beta-lactamase resistance genes identified were primarily species-specific. However, bla NDM-7 and bla CMY-4 were detected in all Escherichia coli and most Providencia rettgeri isolates; bla NDM-7 was also detected in 1 Enterobacter cloacae. The E. coli and E. cloacae isolates also shared bla OXA-1, while bla OXA-10 was found in all P. rettgeri, one Pseudomonas aeruginosa and 1 E. coli. Except for Stenotrophomonas maltophilia isolates, which only contained bla L1 , most species carried multiple beta-lactamase genes, including those encoding extended-spectrum beta-lactamases, AmpC and OXA in addition to a carbapenemase gene. Carbapenemase genes were either class B or class D beta-lactamases. No carbapenemase gene was detected by WGS in 13.6% of isolates.
(Copyright © 2021 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: