Bovine tuberculosis due to Mycobacterium bovis and other mycobacteria among water buffalo (Bubalus bubalis) from the Brazilian Amazon.
| Autor: | Da Silva Pedroso SC; Post-graduate Program in Animal Science, Federal University of Pará, Belém, Brazil., Batista Lima KV; Bacteriology and Mycology Section, Evandro Chagas Institute, Ananindeua, Brazil., Perini Furlaneto I; Post-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil., Corrêa Rodrigues Y; Post-graduate Program in Parasitic Biology in the Amazon, State University of Pará, Belém, Brazil., Saraiva Queiroz Pantoja DK; Post-graduate Program in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Brazil., Souza de Souza AJ; Max Planck University Center, Indaiatuba, Brazil. souzajralex@gmail.com., Assunção Pereira WL; Institute of Animal Health and Production, Federal Rural University of Amazonia, Belém, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of infection in developing countries [J Infect Dev Ctries] 2021 May 31; Vol. 15 (5), pp. 736-741. Date of Electronic Publication: 2021 May 31. |
| DOI: | 10.3855/jidc.13558 |
| Abstrakt: | Introduction: Zoonotic tuberculosis is a disease of public health importance worldwide, especially in developing countries. The present study aimed to investigate the role played by Mycobacterium bovis and other mycobacteria as etiologic agents of bubaline tuberculosis (TB) in the Brazilian Amazon region. Methodology: Granulomatous lesions suggestive of TB obtained from 109 buffaloes (n =109) during sanitary inspection at slaughter were subjected to histopathological evaluation, immunohistochemical (IHC) detection of Mycobacterium antigens, and to molecular tests (PCR) to detect hsp65, IS6110 and RD4 genes, which are specific to Mycobacterium spp., Mycobacterium tuberculosis Complex (MTBC) and M. bovis, respectively. Results: PCR results indicated Mycobacterium infection in 87.2% of the cases, of which 69.5% were positive for M. bovis, 27.4% belonged to MTBC, and 3.1% were probably non-TB mycobacteria. There was good agreement between the genus-specific molecular technique and the histopathological analysis. This high frequency of TB cases caused by non-M. bovis suggests a diversified scenario of mycobacteria associated with bubaline TB in the Brazilian Amazon region. Conclusions: The results reinforce the need of discussing the inclusion of more accurate techniques in examinations carried out by Inspection Services in Brazil. Competing Interests: No Conflict of Interest is declared (Copyright (c) 2021 Silvia Cristina da Silva Pedroso, Karla Valeria Batista Lima, Ismari Perini Furlaneto, Yan Correa Rodrigues, Darlene Kassia Saraiva Queiroz Pantoja, Alex Junior Souza de Souza, Washington Luiz Assuncao Pereira.) |
| Databáze: | MEDLINE |
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