Full genome-based characterization of G4P[6] rotavirus strains from diarrheic patients in Thailand: Evidence for independent porcine-to-human interspecies transmission events.

Autor: Tacharoenmuang R; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand.; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Guntapong R; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Upachai S; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Singchai P; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Fukuda S; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Ide T; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Hatazawa R; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Sutthiwarakom K; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Kongjorn S; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Onvimala N; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Luechakham T; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Ruchusatsawast K; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Kawamura Y; Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Sriwanthana B; Medical Sciences Technical Office, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Motomura K; Thailand-Japan Research Collaboration Center on Emerging and Re-Emerging Infections, Nonthaburi, 11000, Thailand.; Osaka Institute of Public Health, Osaka, 537-0025, Japan., Tatsumi M; Thailand-Japan Research Collaboration Center on Emerging and Re-Emerging Infections, Nonthaburi, 11000, Thailand., Takeda N; Thailand-Japan Research Collaboration Center on Emerging and Re-Emerging Infections, Nonthaburi, 11000, Thailand., Yoshikawa T; Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Murata T; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Uppapong B; National Institute of Health, Department of Medical Sciences, Nonthaburi, 11000, Thailand., Taniguchi K; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan., Komoto S; Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, 470-1192, Japan. satoshik@fujita-hu.ac.jp.
Jazyk: angličtina
Zdroj: Virus genes [Virus Genes] 2021 Aug; Vol. 57 (4), pp. 338-357. Date of Electronic Publication: 2021 Jun 09.
DOI: 10.1007/s11262-021-01851-y
Abstrakt: The exact evolutionary patterns of human G4P[6] rotavirus strains remain to be elucidated. Such strains possess unique and strain-specific genotype constellations, raising the question of whether G4P[6] strains are primarily transmitted via independent interspecies transmission or human-to-human transmission after interspecies transmission. Two G4P[6] rotavirus strains were identified in fecal specimens from hospitalized patients with severe diarrhea in Thailand, namely, DU2014-259 (RVA/Human-wt/THA/DU2014-259/2014/G4P[6]) and PK2015-1-0001 (RVA/Human-wt/THA/PK2015-1-0001/2015/G4P[6]). Here, we analyzed the full genomes of the two human G4P[6] strains, which provided the opportunity to study and confirm their evolutionary origin. On whole genome analysis, both strains exhibited a unique Wa-like genotype constellation of G4-P[6]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The NSP1 genotype A8 is commonly found in porcine rotavirus strains. Furthermore, on phylogenetic analysis, each of the 11 genes of strains DU2014-259 and PK2015-1-0001 appeared to be of porcine origin. On the other hand, the two study strains consistently formed distinct clusters for nine of the 11 gene segments (VP4, VP6, VP1-VP3, and NSP2-NSP5), strongly indicating the occurrence of independent porcine-to-human interspecies transmission events. Our observations provide important insights into the origin of zoonotic G4P[6] strains, and into the dynamic interaction between porcine and human rotavirus strains.
Databáze: MEDLINE
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