A novel chronic wound biofilm model sustaining coexistence of Pseudomonas aeruginosa and Staphylococcus aureus suitable for testing of antibiofilm effect of antimicrobial solutions and wound dressings.

Autor: Chen X; Center for Microbial Communities, Aalborg University, Aalborg East, Denmark., Lorenzen J; Environmental Technology, Danish Technology Institute, Aarhus, Denmark., Xu Y; Center for Microbial Communities, Aalborg University, Aalborg East, Denmark.; Environmental Technology, Danish Technology Institute, Aarhus, Denmark., Jonikaite M; Center for Microbial Communities, Aalborg University, Aalborg East, Denmark., Thaarup IC; Costerton Biofilm Center, University of Copenhagen, Copenhagen, Denmark., Bjarnsholt T; Costerton Biofilm Center, University of Copenhagen, Copenhagen, Denmark.; Department of Clinical Microbiology, Copenhagen University Hospital, Copenhagen, Denmark., Kirketerp-Møller K; Department of Dermatology and Wounds, Bispebjerg and Frederiksberg Hospital, Copenhagen, Denmark., Thomsen TR; Center for Microbial Communities, Aalborg University, Aalborg East, Denmark.; Environmental Technology, Danish Technology Institute, Aarhus, Denmark.
Jazyk: angličtina
Zdroj: Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society [Wound Repair Regen] 2021 Sep; Vol. 29 (5), pp. 820-829. Date of Electronic Publication: 2021 Jun 09.
DOI: 10.1111/wrr.12944
Abstrakt: Chronic wounds are a large burden to patients and healthcare systems. Biofilm infections in chronic wounds are crucial factors leading to non-healing of wounds. It is important to study biofilm in wounds and to develop effective interventions against wound biofilm. This study presents a novel in vitro biofilm model mimicking infected chronic wounds. The novel layered chronic wound biofilm model uses woundlike media and includes both Pseudomonas aeruginosa and Staphylococcus aureus, which have been identified as the most important pathogens in wounds. The model sustains their coexistence for at least 96 h. Microscopy of the model revealed microbial growth in non-surface attached microcolonies as previously observed in vivo. The model was used to determine log 10 -reduction for the use of an antimicrobial solution and antimicrobial dressings (containing silver or honey) showing moderate-to-low antibiofilm effect, which indicates better concordance with the observed clinical performance of this type of treatment than other widely used standard tests.
(© 2021 The Authors. Wound Repair and Regeneration published by Wiley Periodicals LLC on behalf of The Wound Healing Society.)
Databáze: MEDLINE
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