A promiscuous glycosyltransferase generates poly-β-1,4-glucan derivatives that facilitate mass spectrometry-based detection of cellulolytic enzymes.

Autor: Bulmer GS; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Mattey AP; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Parmeggiani F; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk and Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milano, Italy., Williams R; School of Chemistry, University of Bristol, Bristol, UK., Ledru H; School of Chemistry, University of Bristol, Bristol, UK., Marchesi A; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Seibt LS; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Both P; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Huang K; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Galan MC; School of Chemistry, University of Bristol, Bristol, UK., Flitsch SL; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., Green AP; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk., van Munster JM; Manchester Institute of Biotechnology (MIB) & School of Natural Sciences, The University of Manchester, Manchester, UK. jolanda.vanmunster@manchester.ac.uk jolanda.van-munster@sruc.ac.uk and Scotland's Rural College, Central Faculty, Edinburgh, UK.
Jazyk: angličtina
Zdroj: Organic & biomolecular chemistry [Org Biomol Chem] 2021 Jun 30; Vol. 19 (25), pp. 5529-5533.
DOI: 10.1039/d1ob00971k
Abstrakt: Promiscuous activity of a glycosyltransferase was exploited to polymerise glucose from UDP-glucose via the generation of β-1,4-glycosidic linkages. The biocatalyst was incorporated into biocatalytic cascades and chemo-enzymatic strategies to synthesise cello-oligosaccharides with tailored functionalities on a scale suitable for employment in mass spectrometry-based assays. The resulting glycan structures enabled reporting of the activity and selectivity of celluloltic enzymes.
Databáze: MEDLINE