Current Applications and Future Perspectives of CRISPR-Cas9 for the Treatment of Lung Cancer.

Autor:	Tiruneh G/Medhin M; Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia., Chekol Abebe E; Department of Biochemistry, College of Health Sciences, Debre Tabor University, Debre Tabor, Ethiopia., Sisay T; Institute of Biotechnology, College of Natural Science, University of Gondar, Gondar, Ethiopia., Berhane N; Institute of Biotechnology, College of Natural Science, University of Gondar, Gondar, Ethiopia., Bekele T; Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia., Asmamaw Dejenie T; Department of Biochemistry, School of Medicine, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Jazyk:	angličtina
Zdroj:	Biologics : targets & therapy [Biologics] 2021 May 31; Vol. 15, pp. 199-204. Date of Electronic Publication: 2021 May 31 (Print Publication: 2021).
DOI:	10.2147/BTT.S310312
Abstrakt:	Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR-associated proteins are referred to as CRISPR-Cas9. Bacteria and archaea have an adaptive (acquired) immune system. As a result, developing the best single regulated RNA and Cas9 endonuclease proteins and implementing the method in clinical practice would aid in the treatment of diseases of various origins, including lung cancers. This seminar aims to provide an overview of CRISPR-Cas9 technology, as well as current and potential applications and perspectives for the method, as well as its mechanism of action in lung cancer therapy. This technology can be used to treat lung cancer in two different ways. The first approach involves creating single directed RNA and Cas9 proteins and then distributing them to cancer cells using suitable methods. Single directed RNA looks directly at the lung's mutated epidermal growth factor receptor and makes a complementary match, which is then cleaved with Cas9 protein, slowing cancer progression. The second method is to manipulate the expression of ligand-receptors on immune lymphocytic cells. For example, if the CRISPR-Cas9 system disables the expression of cancer receptors on lymphocytes, it decreases the contact between the tumor cell and its ligand-receptor, thus slowing cancer progression. Competing Interests: The authors declare that they have no conflicts of interest for this work. (© 2021 Tiruneh G/Medhin et al.)
Databáze:	MEDLINE
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