Impact of TLR7 and TLR9 polymorphisms on susceptibility to placental infections and pregnancy complications.

Autor: Sánchez-Luquez K; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil. Electronic address: ksanchezluquez@gmail.com., Schadock IC; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Gonçalves CV; Center for Obstetrics and Gynecology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Tornatore M; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Finger-Jardim F; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Avila EC; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Soares MA; Oncovirology Program, National Cancer Institute (INCA), Rio de Janeiro, Rio de Janeiro, Brazil., de Martínez AMB; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil., Ellwanger JH; Laboratory of Immunobiology and Immunogenetics, Department of Genetics, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil., Chies JAB; Laboratory of Immunobiology and Immunogenetics, Department of Genetics, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil., da Hora VP; Laboratory of Molecular Biology, School of Medicine, Universidade Federal do Rio Grande (FURG), Rio Grande, Rio Grande do Sul, Brazil.
Jazyk: angličtina
Zdroj: Journal of reproductive immunology [J Reprod Immunol] 2021 Aug; Vol. 146, pp. 103342. Date of Electronic Publication: 2021 Jun 02.
DOI: 10.1016/j.jri.2021.103342
Abstrakt: This study evaluated the impact of the TLR7 Gln11Leu (rs179008) and TLR9 -1237 T/C (rs5743836) single nucleotide polymorphisms (SNPs) on susceptibility to placental infections and pregnancy complications in 455 Brazilian women. Demographic, socioeconomic, gynecological, and clinical characteristics of the women were collected. Placental tissues were sampled from pregnant women and human and viral DNA was extracted. Human alphaherpesvirus 1 (Herpes simplex virus type 1, HSV-1), Human alphaherpesvirus 2 (Herpes simplex virus type 2, HSV-2) and Human betaherpesvirus 5 (Human cytomegalovirus, HCMV) were detected by nested PCR. TLR9 and TLR7 SNPs were genotyped by PCR amplification of bi-directional specific alleles (Bi-PASA) and restriction fragment length polymorphism (RFLP), respectively. Infections at the time of birth were detected in 45.71 % of women. The presence of the TT genotype (recessive model) of the TLR7 SNP was associated with increased susceptibility to HSV-1 infection (O.R. = 2.23, p = 0.05). The presence of the C allele of the TLR9 SNP, in heterozygosis or homozygosis (dominant model), decreased the infection risk by HCMV (O.R. = 0.31, p-mod<0.05). The TT genotype (recessive model) of the TLR7 SNP was significantly associated (p < 0.05) with increased occurrence of pre-treated hypertension. The codominant model of the TLR9 SNP was significantly associated (p < 0.05) with reduced risk of hospitalization during pregnancy. In combination, the AA/CT (TLR7-TLR9) genotypes significantly decreased the risk of placental infection by HSV-1 and/or HSV-2 (O.R. = 0.47, p = 0.02), the susceptibility to all infectious agents considered in combination (O.R. = 0.4, p = 0.00), and the need of hospitalization (O.R. = 0.48, p = 0.02). In conclusion, TLR7 and TLR9 SNPs are potential modulating factors for the risk of placental infections and pregnancy complications.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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