| Autor: |
Balch HB; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States.; Kavli Energy NanoSciences Institute at the University of California Berkeley and the Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., McGuire AF; Department of Chemistry, Stanford University, Stanford, California 94305, United States., Horng J; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States.; Kavli Energy NanoSciences Institute at the University of California Berkeley and the Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., Tsai HZ; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States., Qi KK; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States., Duh YS; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States., Forrester PR; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States., Crommie MF; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States.; Kavli Energy NanoSciences Institute at the University of California Berkeley and the Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., Cui B; Department of Chemistry, Stanford University, Stanford, California 94305, United States., Wang F; Department of Physics, University of California Berkeley, Berkeley, California 94720, United States.; Kavli Energy NanoSciences Institute at the University of California Berkeley and the Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States.; Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States. |
| Abstrakt: |
The measurement of electrical activity across systems of excitable cells underlies current progress in neuroscience, cardiac pharmacology, and neurotechnology. However, bioelectricity spans orders of magnitude in intensity, space, and time, posing substantial technological challenges. The development of methods permitting network-scale recordings with high spatial resolution remains key to studies of electrogenic cells, emergent networks, and bioelectric computation. Here, we demonstrate single-shot and label-free imaging of extracellular potentials with high resolution across a wide field-of-view. The critically coupled waveguide-amplified graphene electric field (CAGE) sensor leverages the field-sensitive optical transitions in graphene to convert electric potentials into the optical regime. As a proof-of-concept, we use the CAGE sensor to detect native electrical activity from cardiac action potentials with tens-of-microns resolution, simultaneously map the propagation of these potentials at tissue-scale, and monitor their modification by pharmacological agents. This platform is robust, scalable, and compatible with existing microscopy techniques for multimodal correlative imaging. |
